The conifer Larix olgensis has been analyzed to delineate physiological and proteomic changes that occur under drought stress. Studies of the deleterious effects of drought in the larch families have mainly focused on photosynthesis. In the present study, when the intensity of drought was increased, plant height was inhibited as both POD and MDA levels increased, which indicates oxidative stres...

Staphylococcus epidermidis, which is a causative pathogen of nosocomial infection, expresses its virulent traits such as biofilm and autolysis regulated by two-component signal transduction system SaeRS. In this study, we performed a proteomic analysis of differences in expression between the S. epidermidis 1457 wild-type and saeRS mutant to identify candidates regulated by saeRS using two-dime...

Epstein Barr virus infection is believed to play a role in the development of nasopharyngeal carcinoma. In order to investigate the function of EBV in epithelial cell, proteomic methods were used to find and identify the differential proteins and expected to elucidate the mechanism of EBV. Altered protein expressions were found between 293 cell (HEK293) and EBV infected cell (293-EBV). In this ...

BACKGROUND AND OBJECTIVE Protein expression in colon and rectal cancer (CRC) and paired normal tissues was examined by two-dimensional gel electrophoresis (2-DE) to identify differentially expressed proteins. MATERIALS AND METHODS Five fresh colorectal cancer and paired adjacent normal tissues were obtained and differentially expressed protein spots were determined using PDQuest software, wit...

Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a rapidly growing method in the life sciences. However, for many analyte classes, its sensitivity limited due to poor ionization efficiencies. To mitigate this problem, we here introduce novel post-ionization scheme based on single-photon induced chemical using pulsed RF-Kr lamps. The fine-vacuum conditions of ...

With new emerging mass spectrometry technologies, it can now be demonstrated that direct tissue analysis is feasible using matrix-assisted laser desorption/ionization (MALDI) sources. A major advantage of direct MALDI analysis is to avoid time-consuming extraction, purification or separation steps, which have the potential for producing artifacts. Direct MALDI analysis of tissue sections enable...

̨-Cyano-4-hydroxycinnamic acid (CHCA) is often selected as the matrix-assisted laser desorption/ionization (MALDI) matrix for peptide analysis. However, CHCA is recognized as a hot matrix,1–5 which is seldom used in the analysis of phosphopeptides. This is because extensive fragmentation from intact analytes is frequently observed in MALDI mass spectra when CHCA is used as the matrix.6,7 However...

MALDI imaging mass spectrometry represents a new analytical tool to directly provide the spatial distribution and relative abundance of proteins in tissue. Twenty-five ovary carcinomas (stages III and IV) and 23 benign ovaries were directly analyzed using MALDI-TOF MS. The biomarker with the major prevalence (80%) has been fully identified using MALDI MS and nanoESI MS and MS/MS after separatio...

This study was conducted in Côte d’Ivoire to determine the respective prevalence of Candida species and their susceptibility profile antifungal agents identify emergence resistance.This retrospective from 2017 2019 at Institute Pasteur on patients with fungal disease. The yeasts isolated identified were subcultured a chromogenic medium MALDI-TOF-MS. Sensitivity tests performed using CLSI method...