Significance We report an intersubunit interaction within the coronavirus replication–transcription complex that is critical for replication and evolutionarily conserved. provide evidence nsp12-associated NiRAN domain has nucleoside monophosphate (NMP) transferase activity in trans identified nsp9, RNA-binding protein, as its target. catalyzes covalent attachment of NMP moiety to conserved nsp9...

Significance CENP-A, the histone H3 variant that forms a unique centromeric chromatin, is essential for faithful chromosome segregation during mitosis. Inability to connect centromere mitotic spindle causes aneuploidy, hallmark of many cancers. In addition missegregation, fusions at (peri)centromeres are prevalent in cancers, but how such rearrangements arise remains unclear. Here, we identifie...

the ds-rna activated protein kinase (pkr) is a serine-threonine kinase with mw of 68 kda. it belongs to a family of kinases that control one of the translational initiation factors, eif2. pkr is produced at high level in response to viral infection. this protein by phosphorylating eif2 inhibits cellular protein synthesis. in this study, the effect of gamma interferon (ifn-γ), an activator, and ...

Replication initiation, elongation and completion are tightly coordinated to ensure that all sequences replicate precisely once each generation. UV-induced DNA damage disrupts replication delays elongation, which may compromise this coordination leading genome instability cell death. Here, we profiled the Escherichia coli as it recovers from UV irradiation determine how these replicational proc...

Microsatellites or simple sequence repeats (SSRs) are very effective molecular markers in population genetics, genome mapping, taxonomic study and other large-scale studies. Variation in number of tandem repeats within microsatellite refers to simple sequence length polymorphism (SSLP); but there are a few studies that are showed SSRs replication slippage may be occurred during in vitro amplifi...