Helicases are ubiquitous enzymes that unwind double-stranded DNA (dsDNA) to reveal single-stranded DNA (ssDNA) during essential processes such as replication, transcription, or repair. The Escherichia coli RecQ protein is a 3' to 5' helicase, which functions in the processes of homologous recombination and replication fork restart. Here, we analyzed the relationship between ATP hydrolysis by Re...

A kinetic priority queue is a kinetic data structure which determines the largest element in a collection of continuously changing numbers subject to insertions and deletions. Due to its importance, many different constructions have been suggested in the literature, each with its pros and cons. We propose a simple construction that takes advantage of randomization to achieve optimal locality an...

During DNA replication replicative polymerases move in discrete mechanical steps along the DNA template. To address how the chemical cycle is coupled to mechanical motion of the enzyme, here we use optical tweezers to study the translocation mechanism of individual bacteriophage Phi29 DNA polymerases during processive DNA replication. We determine the main kinetic parameters of the nucleotide i...

Since entering the human population, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2; causative agent of Disease 2019 [COVID-19]) has spread worldwide, causing >100 million infections and >2 deaths. While large-scale sequencing efforts have identified numerous genetic variants in SARS-CoV-2 during its circulation, it remains largely unclear whether many these changes impact ad...

The principles that govern the emergence of life from non-life remain a subject of intense debate. The evolutionary paradigm built up over the last 50 years, that argues that the evolutionary driving force is the Second Law of Thermodynamics, continues to be promoted by some, while severely criticized by others. If the thermodynamic drive toward ever-increasing entropy is not what drives the ev...

toxoplasmosis is a protozoal infection caused by toxoplasma gondii. toxoplasmosis produce severe damage in patients who are immunosuppresed. in those who are immunosupressed, latent infection can be reactivated resulting in acute disseminating disease. betamethasone is a synthetic glycocorticoid, used as an anti-inflamatory and immunosuppressant in a wide variety of disorders.the aim of this st...

data grids support access to widely distributed storage for large numbers of users accessing potentially many files. to enhance access time, replication at nearby sites may be used. data replication, a technique much investigated bydata grid researchers in past years creates multiple replicas offile and places them in conventional locations to shorten fileaccess times. one of the problems in da...

Before a cell divides into two daughter cells, its entire genetic material has to be copied without errors and exactly once. In eukaryotic cells, a vast amount of replication origins exists that enable the replication of the DNA to initiate simultaneously from many origins in parallel, thereby contributing to a relatively rapid duplication of the genome. The initiation of DNA replication from t...