background and objective: high-level gentamicin resistance (hlgr: mic ≥ 500 µg/ml) in enterococci is mediated by aminoglycoside modifying enzymes which is mainly encoded by aac(6′)-ie-aph(2′′)-ia gene. the aim of this study was to evaluate the frequency of   aac(6′)-ie-aph(2′′)-ia gene in clinical isolates of enterococcus facium and enterococcus faecalis collected from hospitals in northwest of...

Staphylococcus epidermidis is the most important member of coagulase negative staphylococci responsible for community and hospital acquired infections. Most clinical isolates of S. epidermidis are resistant to methicillin making these infections difficult to treat. In this study, correlation of methicillin resistance phenotype was compared with methicillin resistance (mecA) gene carriage in 55 ...

objective(s): macrolide resistant streptococcus pneumoniae pose an emerging problem globally. the aim of this study was to investigate the prevalence of ermb and mefa genes (macrolide resistant genes) by polymerase chain reaction (pcr) method and to detect drug resistance patterns of s. pneumoniae isolated from clinical samples to macrolides and other antibiotic agents by e-test method. materia...

the green fluorescent protein (gfp) was originally isolated from the jellyfish aequorea victoria that fluoresces green when exposed to blue light. gfp protein is composed of 238 amino acids with the molecular mass of 26.9 kd. the gfp gene is frequently used in cellular and molecular biology as a reporter gene. to date, many bacterial, yeast , fungal, plants, fly and mammalian cells, including h...

background and objective: the production of carbapenemases especially klebsiella pneumoniae carbapenemase (kpc) is the most important mechanism of enzymatic resistance in isolated enterobacteriaceae such as k. pneumoniae. the purpose of this study was detected of the carbapenemase producer k. pneumoniae strains with phenotypic and genotypic methods. method: out of 800 strains, 270 k. pneumoniae...

septoria tritici blotch (stb), caused by the ascomycete fungus mycosphaerella graminicola (asexual stage: septoria tritici), is one of the most important foliar diseases of wheat. in this research, quantitative expression analysis of five candidate genes for the induction of resistance to stb (pr-1, bsi, msr, per, and ppi) was conducted in the wheat cultivars ‘seri 82’ (susceptible) and ‘fronta...

background: for the first time we tested an association between the human multidrug resistance gene 1 (mdr1) polymorphisms (snps) and idiopathic pulmonary fibrosis (ipf). several mdr1 polymorphisms are associated with pathologies in which they modify the drug susceptibility and pharmacokinetics. materials and methods: we genotyped three mdr1 polymorphisms of 48 ipf patients and 100 control subj...

five common tetracycline resistance genes tet(a), tet(b), tet(m), tet(o) and tet(s) were studied by polymerase chain reaction in 100 bacteria isolated from iranian fish farms. in the antibiogram test most of the bacteria were either intermediately or completely resistant to tetracycline. nine isolates out of 46 aeromonas spp. contained either tet(a/m/s) resistant genes as follows: tet(a) in a. ...

background resistance exercise is recommended as a useful therapeutic tool for the treatment of type 2 diabetes (t2d); however, the frequency of studies is inadequate to establish the precise mechanisms of any association between them. objectives in this study, we aimed to assess the effect of three months of resistance training on tcf7l2 expression in pancreatic tissues, serum insulin and gluc...

ABSTRACTTuberculosis is an infectious disease which occurs widely in the world and becomes one of the top 10 causes of death worldwide. Mutations of the RpoB gene cause the resistance of Mycobacterium tuberculosis to rifampicin that contributes to the occurrence of MDR-TB. This study aimed to determine the pattern of rpoB gene polymorphisms in the MDR M. tuberculosis in Semarang, Indonesia. Mos...