Strawberry mottle virus (SMoV) is one of the viral pathogens affecting strawberries (Fragaria spp.) production severely worldwide. Currently, reverse transcription-polymerase chain reaction (RT-PCR) is frequently used to detect SMoV, which is sometimes not successful because of unsatisfied nucleic acid quality and the contamination of secondary metabolites resulting in poor PCR amplification. I...

Because of the allelic variations within the M protein gene (emm gene) of group A streptococci, reliable typing of this important human pathogen can be accomplished by the use of emm gene-specific oligonucleotide probes. Two technical modifications (a reverse dot blot and a reverse line blot hybridization assay) of a novel approach for the type-specific identification of emm genes have been dev...

BACKGROUND Western blot is the gold standard method to determine individual protein expression levels. However, western blot is technically difficult to perform in large sample sizes because it is a time consuming and labor intensive process. Dot blot is often used instead when dealing with large sample sizes, but the main disadvantage of the existing dot blot techniques, is the absence of sign...

AIMS To determine the relative diagnostic performance of non-isotopic in situ hybridisation (NISH) and a dot-blot assay for detecting human papillomavirus (HPV) on exfoliated cervical cells; and to correlate the results with cytopathological assessment. METHODS Cervical smears and cytological samples were obtained from 122 patients during the same clinical examination and the presence of HPV ...