نتایج جستجو برای: reverse staining

تعداد نتایج: 182890  

Journal: :Journal of Clinical Pathology 1980

Journal: :Journal of Clinical Pathology 1996

Journal: :British Dental Journal 2013

Journal: :iranian journal of veterinary research 2014
s. hosseinzadeh s. s. shekarforoush m. fazeli m. dehghan

the objective of the current study was to evaluate the reproducibility of a reverse transcriptase pcr (rt-pcr)-based technique to differentiate viable and dead salmonella cells in raw and sterilized milk. the microorganism was initially inoculated into the milk samples followed by incubating at 37°c for 4 h prior to inactivation by heat at 80°c for 10 min. the treated and non-treated samples we...

Ali Davati Mohammad Hossein Ghaini, Shaghayegh Sadat Esmailnejad

Background and Aims: Immunohistochemical tests are one of the most important tests, which are under study to determine the prognosis of the cancers such as transitional cell carcinoma. By the time, flexible cystoscopy and urine cytology are the routine tests for following up the patients with transitional cell carcinoma, which are both operator dependent. On the other hand, cystoscopy is an inv...

Journal: :Investigative ophthalmology & visual science 2004
Hiroshi Nakamura Frédéric Chiambaretta Joel Sugar Vincent Sapin Beatrice Y J T Yue

PURPOSE To examine the expression of transcription factor Kruppel-like factor-6 (KLF6) in the cornea and lens of mouse eyes throughout developmental stages. METHODS C57BL/6 mice were set up for timed mating. Embryos on embryonic day (E)10.5, E12.5, E15.5, and E18.5 and eyes from mice on postnatal day (P)0, P7, P11, P15, P30, and P60 were collected for immunofluorescence staining. Transcript o...

2013
Pavel Májek Zuzana Riedelová-Reicheltová Klára Pecánková Jan E. Dyr

The time required to visualize proteins using Coomassie Blue dye has been significantly reduced with the introduction of fast staining protocols based on staining with a Coomassie Blue dye solution at boiling temperatures. However, fast stainings suffer from high gel backgrounds, reducing the signal-to-noise ratio and limiting the number of detectable spots in the case of 2D SDS-PAGE. The aim o...

Journal: :Archives of neurology 2002
Yoshihiro Wakayama Takahiro Jimi Masahiko Inoue Hiroko Kojima Makoto Murahashi Toshiyuki Kumagai Sumimasa Yamashita Hajime Hara Seiji Shibuya

BACKGROUND In Duchenne muscular dystrophy (DMD), previous freeze-fracture electron microscopic studies demonstrated that muscle plasma membrane contained markedly decreased numbers of orthogonal arrays. Recent investigations showed that orthogonal arrays were composed of aquaporin 4 (AQP4) molecules, a member of the water channel protein family. OBJECTIVES To study whether the immunostainabil...

2010
Shuya Hara Akihiro Hazama Masao Miyake Takashi Kojima Yasumasa Sasaki Jun Shimazaki Murat Dogru Kazuo Tsubota

PURPOSE To investigate the presence of epithelial sodium channels (ENaC) in rabbit and human conjunctival epithelium and to test the effects of topical amiloride, a potassium-sparing diuretic that blocks the ENaC, on tear quantity in rabbits. METHODS Both healthy normal human and rabbit conjunctival tissues underwent immunohistochemistry staining for ENaC-α and γ subunits as well as for rever...

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