نتایج جستجو برای: reverse transcriptase enzyme

تعداد نتایج: 344010  

Journal: :Science 1974
R J Mayer R G Smith R C Gallo

Particles with the morphology of type C virus have been identified from primate placentas by electron microscopy. A reverse transcriptase (RNA-dependent DNA polymerase) was isolated and purified from microsomal pellets of two fresh placentas of rhesus monkeys in the early stages of gestation. This enzyme was biochemically similar yet immunologically distinct from the reverse transcriptases of k...

Journal: :The Journal of antibiotics 1986
H Okada H Mukai Y Inouye S Nakamura

Reverse transcriptase of RNA tumor viruses plays an important role in the integration of the viral genome into host cell DNA1,2) and specific inhibitors of this enzyme might provide chemotherapeutic agents against infection by retro viruses. In the course of our screening for enzyme inhibitors against reverse transcriptase of avian myeloblastosis virus (AMV), the novel substances, retrostatin3)...

Journal: :Current HIV research 2004
Nicolas Sluis-Cremer N Alpay Temiz Ivet Bahar

Nonnucleoside reverse transcriptase inhibitors (NNRTI) are a group of small hydrophobic compounds with diverse structures that specifically inhibit HIV-1 reverse transcriptase (RT). NNRTIs interact with HIV-1 RT by binding to a single site on the p66 subunit of the p66/p51 heterodimeric enzyme, termed the NNRTI-binding pocket (NNRTI-BP). This binding interaction results in both short-range and ...

Journal: :BioTechniques 2003
Phillip R Hawkins Pei Jin Glenn K Fu

Reverse transcription of mRNA often leads to the synthesis of partial, non-full-length cDNAs. Methods to facilitate reverse transcription across RNA regions of secondary structure, as well as enzyme modifications to eliminate RNase H activities inherent to reverse transcriptase enzymes, have been previously reported. However, because all reverse transcriptases have high error rates of polymeriz...

Journal: :The Journal of biological chemistry 1992
D C Orr H T Figueiredo C L Mo C R Penn J M Cameron

Carbocyclic 2',3'-didehydro-2',3'-dideoxyguanosine (carbovir, NSC 614846) is an anti-retroviral agent that may be useful in the treatment of AIDS. We have examined the ability of (-)-enantiomeric carbovir triphosphate to inhibit human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (EC 2.7.7.49). A comparison of inhibition kinetics was made with 3'-azido-2',3'-dideoxythymidine triph...

Journal: :Clinical microbiology reviews 1988
J B Jackson H H Balfour

Since the discovery of human immunodeficiency virus (HIV) as the causative agent of acquired immunodeficiency syndrome in 1983, there has been a proliferation of diagnostic tests. These assays can be used to detect the presence of HIV antibody, HIV antigen, HIV ribonucleic and deoxyribonucleic acids, and HIV reverse transcriptase. Enzyme-linked immunosorbent assays, Western blot, radioimmunopre...

Journal: :Indian journal of biochemistry & biophysics 2011
Jian Sun Hexiang Wang Tzi Bun Ng

A laccase with a molecular mass of 67 kDa and inhibitory activity toward HIV-1 reverse transcriptase (IC50 = 7.5 microM) was isolated from fresh fruiting bodies of the Lentinus edodes (Shiitake mushroom). Its characteristics were compared with those of laccases from cultured mushroom mycelia reported earlier. The laccase was unadsorbed on DEAE-cellulose, Affi-gel blue gel and CM-cellulose, but ...

2018
David Baltimore

David Baltimore studied viruses and the immune system in the US during the twentieth century. In 1975, Baltimore was awarded the Nobel Prize in Physiology or Medicine for discovering reverse transcriptase, the enzyme used to transfer information from RNA to DNA. The discovery of reverse transcriptase contradicted the central dogma of biology at the time, which stated that the transfer of inform...

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