The citrus systemic diseases, including Huanglongbing (caused by Candidatus Liberibacter asiaticus (CLas)), tristeza virus (CTV)), tatter leaf (CTLV)), and exocortis viroid (CEVd)), are threats to production in Taiwan. Reliable diagnostic methods important for the management of these diseases. In this study, we developed a multiplex reverse transcription–polymerase chain reaction (RT-PCR) assay...

Objectives: To compare the performance of chest CT and Real-Time Reverse Transcription-Polymerase Chain Reaction (RTPCR) in initial diagnostic assessment coronavirus disease COVID-19 patients during pandemic. Methods& Results:In this study we have included 209 clinically suspected cases COVID-19from Government Medical College, Nagpur who undergone both HRCT RT-PCR. In our study, 3.35% were ...

Background: COVID-19 has so far affected millions of people in India. The present study was undertaken to find out the performance and reliability rapid antigen test (RAT) compared reverse transcription polymerase chain reaction (RT-PCR).Methods: pre existing medical conditions clinical signs symptoms were noted. nasopharyngeal swab samples taken for RAT, while both oropharyngeal mixed a steril...

We evaluated a highly sensitive quantitative real-time one-step reverse transcription-PCR (RT-PCR) for detection of human cytomegalovirus pp67 transcripts in monitoring of solid-organ transplant recipients. Results were compared with those of pp65 antigen testing and quantitative DNA-PCR. Due to a low clinical sensitivity, the pp67 RT-PCR was not able to replace these assays.

The aim of this study was to produce a sensitive, simple and rapid diagnostic method for the detection of foot-and-mouth disease virus (FMDV) in suspected cases of FMD, a one-step single-tube method of reverse transcription loop-mediated isothermal polymerase amplification (RT-LAMP). A set of six common primers was designed to target the highly conserved region of the RNA polymerase 3Dpol gene ...

To investigate the distribution of tumour cells expressing the SYT-SSX fusion gene in biphasic synovial sarcoma, modified reverse transcription polymerase chain reaction (RT-PCR) analysis was performed using microdissected specimens from haematoxylin and eosin stained sections of archival paraffin wax embedded tissues. This modified RT-PCR included a stage with degenerate oligonucleotide primed...

This review presents the basic principles of application loop-mediated isothermal amplification (LAMP) reaction for rapid diagnosis coronavirus infection caused by SARS-CoV-2. The technical details method, and most popular approaches specific non-specific detection products are briefly described. We also discuss first published works on use method nucleic acid SARS-CoV-2 virus, including those ...

We developed a useful method for the detection of rubella virus genome RNA by reverse transcription loop-mediated isothermal amplification (RT-LAMP) and compared the sensitivity of RT-LAMP with that of other virological tests: reverse transcription-PCR (RT-PCR) and virus isolation. The rubella virus genome was amplified by RT-LAMP from clinical isolates obtained between 1987 and 2004 with simil...

A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (RT-nPCR) procedure that identifies the North American strain of WN virus directly in equine and av...

........................................................................................................................................... IX 1. GENERAL INTRODUCTION .......................................................................................................... 1 2. LITERATURE REVIEW .......................................................................................................