abstract: potato virus y a type species of the genus potyvirus infects several crops in the family solanaceae. pvy isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. the result of elisa, immunoblot electrophoresis and immuno capture reverse transcription polymerase chain reaction   (ic-rt-pcr) indicated that pepper isol...

in this study, the possible infection of thomson navel, moro blood sweet orange, page mandarin and webber pomelo with citrus viroids was investigated. biological indexing was performed by grafting on etrog citron (c. medica) and maintained in temperature-controlled greenhouse. after inoculation, rna was extracted by sds– potassium acetate method. detection of citrus viroids was done reverse tra...

This is the first report on circulating canine rotavirus in Mexico. Fifty samples from dogs with gastroenteritis were analyzed used polymerase chain reaction and reverse transcription polymerase chain reaction in order to identify parvovirus and rotavirus, respectively; 7% of dogs were infected with rotavirus exclusively, while 14% were co-infected with both rotavirus and parvovirus; clinical s...

Comparison in virus-seeded mineral water of three detection methods for enteroviruses, direct hybridization, cell culture, and reverse transcription into cDNA followed by polymerase chain reaction and hybridization, showed that the last procedure was 10 to 1,000 times more sensitive than detection by cell culture and 10(5) to 10(7) times more sensitive than direct hybridization. The presence of...

background and aims: influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. a variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. methods: nasal and pharyngeal samples taken from patients were inoculated into madin-darby canine kidney (mock) cells and embryonated chicken eggs (eces). the culture media was as...