PCR-restriction fragment length polymorphism (RFLP)-based analysis, also known as cleaved amplified polymorphic sequence (CAPS), is a popular technique for genetic analysis. It has been applied for the detection of intraspecies as well as interspecies variation. There exist several techniques that are related with PCR-RFLP and also involve gel electrophoresis including techniques for DNA finger...

BACKGROUND AND AIMS The genetic trait plays a part in the pathogenesis of peptic ulcer disease. To identify a DNA marker for peptic ulcer disease, the association between the restriction fragment length polymorphism (RFLP) of the pepsinogen C (PGC) gene and peptic ulcer disease was investigated. PATIENTS AND METHODS One hundred and seventy seven unrelated controls, 75 patients with gastric ul...

Two polymorphic marker genes, merozoite surface protein 3α (PvMSP3α) and merozoite surface protein 3β (PvMSP3β), from 100 Plasmodium vivax field isolates, were investigated using polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP). Genotyping of PvMSP3α and PvMSP3β revealed marked polymorphisms in length and sequence. Three major types of PvMSP3α (Type A, B and C) ...

The aims of the present study were to propose apolymerase chain reaction-restriction fragment length polymorphismPCR-RFLP genotyping method for the AY_376689:c.773C>T single nucleotide polymorphism SNP of the equine PRKAG3 gene, related to muscle performance, as well as to characterize this SNP and a second polymorphism, AAWR_02017454:g.121684T>C of the SPATA1 gene, related to male fertility, i...

Ascaridoid nematodes were found in viscera of the blue whiting Micromesistius poutassou (Risso, 1826) from Aegean Sea parasitological examination. Collected morphologically identified as third (L3) stage Anisakis type I larvae and then subject to Restriction Fragment Length Polymorphism (RFLP) analysis internal transcribed spacer (ITS) region identify species. Randomly selected molecularly conf...

The article presents the results of complex research that continues previous work aimed at determining polymorphism CSN2, PRL, LEP, and TNF-α loci analyzing productivity dairy cattle with different genotypes for these loci. goal is to analyze selection populations cows based on typing individuals allelic variants loci, which are associated economically valuable traits but not directly evaluated...

OBJECTIVES: Genetic susceptibility to cervical cancer in relation transforming growth factor beta 1 (TGFB1) gene polymorphisms has not been investigated extensively among the women Bangladesh. So, aim of this study was find out correlation TGFB1 C509T (rs1800469) and T869C (rs1800470) with risk Bangladeshi women. STUDY DESIGN: 134 patients 102 age-sex matched healthy controls were included from...

Oxidative stress due to free radicals is involved in the pathogenesis of many chronic disorders including cancer, inflammation and neurological diseases. Reactive oxygen species (ROS) may be implicated in age-related macular degeneration (AMD).This study investigated the expression of manganese superoxide dismutase MnSOD on mRNA and protein level. Moreover it examines the genetic sequence polym...

Genetic polymorphism G6721T (rs.7003908) in gene encoding DNA-dependent protein kinase (DNA-PKcs, encoded by the XRCC7 gene) has been defined. In order to get more insight into the genetic structure of Iranian population the present study was carried out on Iranian Persian population living in Shiraz (Fars province, southwest Iran). The total study subjects consisted of 935 (195 males, 740 fema...

Previous studies suggested that genetic polymorphisms of serum amyloid A (SAA) were associated with carotid atherosclerosis. However, the relationship between genetic polymorphisms of SAA and myocardial infarction (MI) remains unclear. In the present study, we analyzed a polymorphism (rs12218) in the SAA1 gene in 840 MI patients and 840 healthy subjects by polymerase chain reaction-restriction ...