Cell-free HIV RNA in plasma was detected and quantitated after antiviral therapy by the polymerase chain reaction. RNA was extracted from plasma, reverse transcribed to cDNA, amplified by polymerase chain reaction, and quantitated by absorbance based on an enzyme-linked affinity assay. 72 HIV antibody-positive subjects had one plasma sample taken. 39 who were not receiving antiretroviral therap...

We investigate a correlation between genetic diversity of hepatitis C virus population and the level of viral RNA accumulation in patient blood. Genetic diversity is defined as the mean Hamming distance between all pairs of virus RNA sequences representing the population. We have found that a low Hamming distance (i.e. low genetic diversity) correlates with a high RNA level; symmetrically, high...

background: rna interference (rnai) is a phenomenon uses double-stranded rna (dsrna) to specifically inhibit gene expression. the non-specific silencing caused by interferon response to dsrna in mammalian cells limits the potential of utilizing rnai to study gene function. duplexes of 21-nucleotide short interfering dsrna (sirna) inhibit gene expression by rnai. in some organisms, sirna can als...

On March 11, 2020, the World Health Organization declared coronavirus disease 2019 (COVID-19) a pandemic; from that date, vaccine race has begun, and many technology platforms to develop specific effective COVID-19 have been launched in several clinical trials (protein subunit, RNA-based, DNA-based, replicating viral vector, nonreplicating inactivated virus, live attenuated virus-like particle)...

background: hepatitis c virus (hcv) infection is the most common transfusion transmitted disease in poly-transfused patients worldwide. in this study we aimed to evaluate the effects of pegylated interferon alfa-2a (peg-ifn a-2a) in reducing serum alt and eradicating serum hepatitis c virus (hcv) rna in hcv infected polytransfused thalassemic patients. materials and methods: a cohort of 51 hcv-...

Dengue is potentially a life-threatening arthropod-borne viral infection for which there are no known therapeutic agents till date. Early stage diagnosis of dengue still lacking. Diagnosis only made after severe manifestations and later stages infection. Timely prognosis can prevent related mortalities. The nucleic acid-based therapy has potential to emerge as promising approach early treatment...

Gene therapy is one of the most attractive fields in tumor therapy. In past decades, significant progress has been achieved. Various approaches, such as viral and non-viral vectors and physical methods, have been developed to make gene delivery safer and more efficient. Several therapeutic strategies have evolved, including gene-based (tumor suppressor genes, suicide genes, antiangiogenic genes...

We report the design of AIE fluorogen (AIEgen)-based multifunctional organic nanoparticles with surface siRNA decoration for targeted photodynamic therapy and RNA interference therapy.

objectives: findings demonstrated that parent of children with cerebral palsy experience elevated level of distress, depression, anxiety, posttraumatic stress symptom and subjective symptom of stress. depression is a common condition that typically has a relapsing course. effective interventions targeting relapse have the potential to dramatically reduce the point prevalence of the condition. m...