Homologous disruption of the murine gene encoding the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) leads to the loss of cAMP-mediated ion transport. Mice carrying this gene defect exhibit meconium ileus at birth and gastrointestinal plugging during the neonatal period, both contributing to high rates of mortality. We investigated whether infectious mammalian rotavirus, the re...

Canine rotavirus was isolated from feces of a Korean Jindo dog with mild diarrhea, and the isolate was genetically characterized. Rotaviral antigen was detected in the feces using a commercial rotavirus antigen detection kit and cytopathic effects were observed in a cell line inoculated with the feces. The virus isolate (GC/KS05) was identified as subtype G3P[3] using reverse transcription poly...

conclusions these results indicate that a. kellalensis extracts exert potent anti-rotaviral activity only after viral adsorption. the two extracts from a. kellalensis showed a good selectivity index. also these results suggest that extracts prepared from the flowers of a. kellalensis may be potential anti-rotaviral agents in vivo and be useful in veterinary medicine. background achillea kellale...

rotavirus is a common infection causing 450,000 deaths annually primarily in children 5 years and below. despite the high burden of disease, little is known about the epidemiology of rotavirus in libya. the aim of this study was to estimate the rotavirus disease burden among libyan children.a cross-sectional study was carried out prospectively among children 5 years old and below between august...

Seven rotavirus strains were isolated in cell cultures from the intestinal contents of piglets with diarrhea. MA104 cells with pancreatin in the cell culture medium was the host system of choice for virus isolation and replication. A cell culture immunofluorescence test in which MA104 cells were used in microtiter plates was very effective for detecting and assaying rotaviruses. A plaque reduct...

Background. Childhood diarrhoea is usually caused by viral or bacterial infection. Rotaviruses are the most com− mon cause of severe diarrhoea in children worldwide and infect virtually children younger than 5 years of age. According to the Polish data, the analysis of etiological factors of acute diarrhoeas in children, shows the predom− inance of rotavirus and Salmonella as causative organism...

Globally, rotaviral vaccines in use today have contributed to the reduction of the incidence of rotaviral diarrhoeas. Despite the substantial protection conferred by the current vaccines against the rotaviral strains, it is only prudent to recognise that other protective factors, like breastfeeding, also provide some degree of protection against this disease. This article has attempted to revie...

The root of Rubia cordifolia has been used traditionally as a hemostatic agent, while the aerial part of the plant consisting of leaf and stem is known to exhibit anti-diarrheal properties and has been widely used as a remedy in many parts of China. As rotavirus is one of the most commonly associated diarrhea-causing pathogen, this study aims to investigate the anti-rotaviral effect of R. cordi...