an endochitinase gene (chit36) was isolated from the biocontrol fungus trichoderma atroviride. chit36 was overexpressed under the camv35s constitutive promoter in canola. transformation of cotyledonary petioles was achieved via agrobacterium tumefaciens. the insertion of transgene was verified by pcr and dna dot blotting. rt-pcr analysis indicated that the transgenic canola was able to express ...

Sclerotinia sclerotiorum is an important pathogen of numerous crops in the North Central region of the United States. The objective of this study was to examine the genetic diversity of 145 isolates of the pathogen from multiple hosts in the region. Mycelial compatibility groups (MCG) and microsatellite haplotypes were determined and analyzed for standard estimates of population genetic diversi...

The apparent digestibility coefficients (ADC) of unprocessed and processed pea, canola and flax products were determined on diets, in which 30% of a reference diet was replaced by each test ingredient. Celite was used as an indigestible marker for measuring apparent digestibility coefficients indirectly. The trial was conducted in a semi-closed recirculating system using 5 tanks per diet with 4...

sclerotinia stem rot (white stem rot), an important fungal disease, which is caused by sclerotinia sclerotiorum, is one of the serious diseases of various plants such as canola. use of resistant cultivars is an effective method to control the disease. a greenhouse experiment based on cotyledon inoculation using mycelial suspension of two different isolates of s. sclerotiorum was conducted to ev...

Background: Canola is an agro-economically oilseed crop. Yield loss due to fungal disease of stem rot caused by Sclerotinia sclerotiorum is a serious problem in canola cultivation. Thaumatin-like proteins are large groups of the pathogenesis-related proteins which provide resistance to the fungal infection in response to invading pathogens and play a key role in plant defense s...

The objective of this study was to examine the effects of dietary forage level and fat supplementation on the chemical composition of mixed ruminal bacteria (MRB). Six ruminally cannulated beef steers (354 kg +/- 18) were given ad libitum access to six diets (13.2% CP; DM basis) that were offered twice daily in a 6 x 6 Latin square design. Treatments were arranged as a 2 x 3 factorial with two ...

in order to evaluate competition among some weed species and canola, a field experiment in astan ghods razavi's field in mashhad was done on 2007-2008. thirty 50×50 cm quadrates were used in two times (stem and flower production) to measure the canola dry weight, leaf area index and canola and weeds density with destruction and thirty similar quadrates were used for counting weed species a...

Canola (Brassica napus L.) is commonly used as a hyper-accumulator for phytoextraction of heavy metals from soil and water. Like many other heavy metals, lead (Pb) contaminates soil, water and air and thus it is a great problem. This study was conducted to investigate toxic effects of Pb on growth and nutrient uptake in four canola cultivars. Each of four cultivars of canola (Con-II, Con-III, L...

The development of low erucic acid, low glucosinolate cultivars of canola seed has led to the availability of a feed ingredient with considerable potential to replace soyabean meal in diets for all classes of farm animals. The sinapine and glucosinolate content of various canola cultivars cultivated in two areas of the Western Cape, South Africa were compared. There were no significant differen...