نتایج جستجو برای: sag1

تعداد نتایج: 216  

ژورنال: :مجله علوم پزشکی رازی 0
کامی حسینیان خسروشاهی k hosseinian khosroshahi ms in parasitology. فاطمه غفاری فر f ghaffarifar assistant professor of parasitology. faculty of medicine. tarbiat modarres university. tehran, iran زهره شریفی z sharifi assistant professor of virology. blood transfusion organization. research center of virology. tehran, iran. عبدالحسین دلیمی اصل a.h dalimi asl professor of parasitology. faculty of medicine. tarbiat modarres university. tehran, iran. کاوس صلح جو k solhjo phd student of parasitology. faculty of medicine. tarbiat modarres university. tehran, iran.

زمینه و هدف: بیماری توکسوپلاسموزیس توسط تک یاخته انگلی به نام توکسوپلاسماگوندی(toxoplasma gondii) ایجاد می شود.sag1(surface antigen 1)، آنتی ژن اختصاصی ـ مرحله ای است که فقط در مرحله تاکی زوئیت وجود دارد و در مراحل اسپوروزئیت و برادی زوئیت وجود ندارد و به مقدار فراوان و به طور یکنواخت بر روی سطح داخل و خارج سلولی تاکی زوئیت ها توزیع شده است. این آنتی ژن دارای دو گلیکوفرم است و آنتی ژن، کاملاً co...

2012
M (Mina) Selseleh H Keshavarz M Mohebali S Shojaee MH Modarressi MR Eshragian M (Monavar) Selseleh

BACKGROUND The assays currently available for the detection of specific anti-Toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the Lack of a purified standardized antigen. The aim of this study was evaluation the recombinant Toxoplasma gondii SAG1 antigen for the serodiagnosis of acute and chronic toxoplasmosis. METHODS This study describes an ELISA usi...

Journal: :iranian journal of parasitology 0
m (mina) selseleh dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran h keshavarz dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran and center for research of endemic parasites of iran (crepi), tehran university of medical sciences, tehran, iran m mohebali dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran s shojaee dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran mh modarressi genetic faculty, school of public health, tehran university of medical sciences, tehran, iran mr eshragian epidemiology and biostatistics department, school of public health, tehran university of medical sciences, tehran, iran

background: the assays currently available for the detection of specific anti- toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the lack of a purified standardized antigen. the aim of this study was evaluation the recombinant toxoplasma gondii sag1 antigen for the serodiagnosis of acute and chronic toxoplasmosis. methods : this study describes an elisa u...

Journal: :archives of clinical infectious diseases 0
seyedeh mahya marashiyan department of basic sciences, sari agricultural sciences and natural resources university, sari, iran fatemeh moradian department of basic sciences, sari agricultural sciences and natural resources university, sari, iran geita saadatnia department of biotechnology, iranian research organization for science and technology (irost), tehran, iran; department of biotechnology, iranian research organization for science and technology (irost), tehran, iran. tel/fax: +98-2156276636 majid golkar molecular parasitology laboratory, department of parasitology, pasteur institute of iran, tehran, iran

results the results showed that sensitivity and specificity were respectively 84.12% and 92% for‎ recombinant protein srs3 and 92% and 96% for rsag1. conclusions recombinant proteins srs3 and rsag1 are promising antigens in diagnostic assays for detection of specific antibodies against t. gondii. background infections by the protozoan parasite toxoplasma gondii (t. gondii) are widely prevalent ...

Journal: :Journal of clinical microbiology 2003
Elisa Beghetto Wilma Buffolano Andrea Spadoni Mariassunta Del Pezzo Manlio Di Cristina Olga Minenkova Eskild Petersen Franco Felici Nicola Gargano

The objective of this work was to develop an antibody-specific immunoglobulin G (IgG) avidity assay to discriminate between acute and latent phases of Toxoplasma gondii infection by using recombinant antigens. One hundred twenty-one serum samples from women who developed IgG antibodies against Toxoplasma during pregnancy were used. The IgG avidities of antibodies directed against epitopes carri...

2018
Zineb Lakhrif Alexis Moreau Bruno Hérault Anne Di-Tommaso Matthieu Juste Nathalie Moiré Isabelle Dimier-Poisson Marie-Noëlle Mévélec Nicolas Aubrey

Toxoplasmosis is a major public health problem and the development of a human vaccine is of high priority. Efficient vaccination against Toxoplasma gondii requires both a mucosal and systemic Th1 immune response. Moreover, dendritic cells play a critical role in orchestrating the innate immune functions and driving specific adaptive immunity to T. gondii. In this study, we explore an original v...

Journal: :The Journal of parasitology 2009
J P Dubey J Mergl E Gehring N Sundar G V Velmurugan O C H Kwok M E Grigg C Su D Martineau

Toxoplasma gondii infection in marine mammals is intriguing and indicative of contamination of the ocean environment and coastal waters with oocysts. Toxoplasma gondii infection was detected in captive marine mammals at a sea aquarium in Canada. Antibodies to T. gondii were found in all 7 bottlenose dolphins (Tursiops truncatus) tested. Two of these dolphins, as well as a walrus (Odobenus rosma...

2007
Young-Ha Lee Dae-Whan Shin Jae-Ho Lee Ho-Woo Nam Myoung-Hee Ahn

PURPOSE Surface antigen 3 (SAG3) of Toxoplasma gondii is very similar in structure to the major surface antigen 1 (SAG1). Although numerous studies have supported the importance of SAG1 in protection against T. gondii infection, few reports exist on SAG3. MATERIALS AND METHODS Glutathione-S-transferase (GST)-fused SAG3 of T. gondii (rSAG3) were immunized into BALB/c mice alone or in combinati...

Journal: :International Journal of Current Microbiology and Applied Sciences 2017

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهرکرد - دانشکده دامپزشکی 1390

چکیده توکسوپلاسماگوندئی، یک انگل داخل سلولی است، که قدرت آلوده کردن اکثر مهره داران خونگرم را دارا می باشد. گربه سانان مخصوصاً گربه های اهلی، مهم ترین مخزن این تک یاخته برای انسان و دیگر حیوانات به شمار می روند. توکسوپلاسما گوندئی تنوع ژنتیکی بالایی دارد. روش های تشخیصی متعددی جهت تشخیص این عفونت طراحی شده اند که از بین آن ها، روش الایزای مبتنی بر آنتی ژن خالص شده، به لحاظ سهولت، کمی بودن و امک...

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