We are reporting here a new technology for the straightforward production of integrated microarrays. The approach is based on the use of adhesive supports enabling (i) the immobilization of biomolecules as microarrays (up to 2500 spots per cm(2)) and (ii) the easy assembly of these microarrays with complex 3D structures such as 96-well bottomless microplates or polymer and glass microfluidic ne...

Five different immunoassay formats were examined for their ability to detect a minute quantity of prey remains in predator guts. The convergent lady beetle, Hippodamia convergens Guérin-Meneville, that had consumed either one or five pink bollworm, Pectinophora gossypiella (Saunders), eggs was evaluated by the following immunoassays: three variations of enzyme-linked immunosorbent assay (ELISA)...

The sandwich hybridisation assay (SHA) is a DNA probe-based method for rapid identification and enumeration of toxic micro-algae which uses species specific oligonucleotide probes targeted at ribosomal RNA. It is suited to fragile micro-algal cells which commonly collapse during the fixation stage of sample collection, compromising identification by traditional microscopy. The assay has been av...

A novel magnetic nanoparticle-based electrochemical immunoassay of carcinoembryonic antigen (CEA) was designed as a model using CEA antibody-functionalized magnetic beads [DNA/Fe(3)O(4)/ZrO(2); Fe(3)O(4) (core)/ZrO(2) (shell) nano particles (ZMPs)] as immunosensing probes. To design the immunoassay, the CEA antibody and O-phenylenediamine (OPD) were initially immobilized on a chitosan/nano gold...

BACKGROUND We compared the performance of two new commercial tests for the detection of dengue NS1 protein during the clinical phase of dengue virus (DENV) infection-an immunochromatographic test allowing rapid detection of the NS1 antigen, Dengue NS1 Ag STRIP (Bio-Rad Laboratories - Marnes La Coquette, France), and a two-step sandwich-format microplate enzyme-linked immunosorbent assay (ELISA)...

Vibrio tubiashii has been linked to disease outbreaks in molluscan species, including oysters, geoducks, and clams. In particular, oyster hatcheries in the Pacific Northwest have been plagued by intermittent vibriosis since 2006. Accurate detection of vibrios, including V. tubiashii, is critical to the hatcheries in order to allow for rapid remediation efforts. The current methods for detection...

A graph G = (V,E) is a chordal probe graph if its vertices can be partitioned into two sets, P (probes) and N (non-probes), where N is a stable set and such that G can be extended to a chordal graph by adding edges between non-probes. We give several characterizations of chordal probe graphs, first, in the case of a fixed given partition of the vertices into probes and non-probes, and second, i...

A graph G s = (V , E s) is a sandwich for a pair of graphs G t = (V , E t) and G = (V , E) if E t ⊆ E s ⊆ E. A sandwich problem asks for the existence of a sandwich graph having an expected property. In a seminal paper, Golumbic et al. [Graph sandwich problems, J. Algorithms 19 (1995) 449–473] present many results on sub-families of perfect graphs. We are especially interested in comparability ...

Lateral flow tests (LFTs) are an ingenious format for rapid and easy-to-use diagnostics, but they are fundamentally limited to assay chemistries that can be reduced to a single chemical step. In contrast, most laboratory diagnostic assays rely on multiple timed steps carried out by a human or a machine. Here, we use dissolvable sugar applied to paper to create programmable flow delays and prese...

We present a new application of the noncompetitive phage anti-immunocomplex assay (PHAIA) by converting an existing competitive assay to a versatile noncompetitive sandwich-type format using immunocomplex binding phage-borne peptides to detect the brominated flame retardant, brominated diphenyl ether 47 (BDE 47). Three phage-displayed 9-mer disulfide-constrained peptides that recognize the BDE ...