the satellite cells. Contaminating CD45+/Mac-1+/Sca-1+ cells were subsequently removed by MACS cell separation. Cell cycle analysis revealed that over 80% of the resulting myogenic cell population was in G0, indicating a high proportion of quiescent cells in the isolated population. RT-PCR analysis showed that the expression of three Kruppel-like factors (Klf) was increased in quiescent satelli...

Powerful genetic tools in classical laboratory models have been fundamental to our understanding of how stem cells give rise to complex neural tissues during embryonic development. In contrast, adult neurogenesis in our model systems, if present, is typically constrained to one or a few zones of the adult brain to produce a limited subset of neurons leading to the dogma that the brain is primar...

The problem of regeneration is fundamentally a problem of tissue homeostasis involving the replacement of cells lost to normal 'wear and tear' (cell turnover), and/or injury. This attribute is of particular significance to organisms possessing relatively long lifespans, as maintenance of all body parts and their functional integration is essential for their survival. Because tissue replacement ...

(Shaked etal., PLoS ONE 2008). To further investigate this finding, several different approaches have been taken. First, the expression of HDAC-regulated candidate genes responsible for controlling neurogenesis has been examined through quantitative realtime RT-PCR. TSA-based inhibition of HDAC activity resulted in upregulation of Bmp2, Stat1, Stat3, Ngn1 and Ngn2 in neural precursors from both...

The planarian Schmidtea mediterranea is a well-studied model organism for developmental research, because of its stem cell system. This characteristic also provides a unique opportunity to study stress management and the effect of stress on stem cells. In this study, we characterised the stress signature at different levels of biological organization. The carcinogenic metal cadmium was used as ...

PURPOSE Following decapitation, the planarian Schmidtea mediterranea regenerates its head and eyes. The gene ovo is required for eye maintenance and regeneration in response to wounding. In this study, we investigated whether eye regeneration in S. mediterranea could occur absent a wound healing response. METHODS One hundred twenty S. mediterranea were treated with ovo RNA interference (RNAi)...

Here we report N(2)O emission results for freshwater marshes isolated from human activities at the Sanjiang Experimental Station of Marsh Wetland Ecology in northeastern China. These results are important for us to understand N(2)O emission in natural processes in undisturbed freshwater marsh. Two adjacent plots of Deyeuxia angustifolia freshwater marsh with different water regimes, i.e., seaso...