Seed storage proteins of grain crops meet the major dietary protein requirement of over half of the world population. However, seed proteins in general are deficient in some essential amino acids and hence are of poor nutritional quality. Therefore, intensive research is going on to isolate and characterize these proteins and their genes, and to produce transgenic crop plants with modified seed...

The analysis of plant proteins has a long and distinguished history, with work dating back over 250 years. Much of the work has focused on seed proteins, which are important in animal nutrition and food processing. Early studies classified plant proteins into groups based on solubility ('Osborne fractions') or protein function. More recently, families have been defined based on stuctural and ev...

Potential involvement of seed storage proteins in susceptibility to aflatoxin contamination was assessed with in vitro tests. Initially, two oilseed storage proteins [cottonseed storage protein (CSP) and zein] were compared with bovine serum albumin (BSA) and collagen. Supplementation of a complete defined medium with either oilseed storage protein resulted in significantly more aflatoxin produ...

Seed-type vacuolar processing enzyme (VPE) activity is predicted to be essential for post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds. To test this hypothesis, we examined the protein profiles of developing and germinating seeds from Arabidopsis plants containing transposon-insertional knockout mutations in the genes that encode the two ...

Most plants synthesise proteins in their organs of reproduction and propagation, such as seeds of gymnosperms and angiosperms. Storage proteins are usually located in two tissues. In dicotyledonous plants they may be located in the diploid cotyledons (exalbuminous}, in the triploid endosperm (albuminous) or, occasionally, in both tissues. In monocotyledonous cereals they are primarily located i...

UNLABELLED Soybeans are an important legume crop that contain 2 major storage proteins, β-conglycinin and glycinin, which account about 70- 80% of total seed proteins. These abundant proteins hinder the isolation and characterization of several low abundant proteins in soybean seeds. Several protein extraction methodologies were developed in our laboratory to decrease these abundant storage pro...

Soybean somatic embryos have attracted attention both as a model of zygotic embryos and as explants for the generation of stable transgenic plants. We have now characterized the maturation of soybean somatic embryos in detail by examining both the accumulation of the major seed storage proteins b-conglycinin and glycinin as well as changes in cellular organization. Protein storage vacuoles and ...

The protein subunit is the most important basic unit of protein, and its study can unravel the structure and function of seed storage proteins in faba bean. In this study, we identified six specific protein subunits in Faba bean (cv. Qinghai 13) combining liquid chromatography (LC), liquid chromatography-electronic spray ionization mass (LC-ESI-MS/MS) and bio-information technology. The results...