we have studied the changes in protein-protein interaction network of 38 different tissues of the human body. 123 gene expression samples from these tissues were used to construct human protein-protein interaction network. this network is then pruned using the gene expression samples of each tissue to construct different protein-protein interaction networks corresponding to different studied ti...

Abstract The detection of intracellular nucleic acids is a fundamental mechanism host defense against infections. dysregulated acid sensing, however, major cause for number autoimmune diseases. In this study, we report that GTPase-activating protein SH3 domain-binding 1 (G3BP1) critical both DNA- and RNA-induced immune responses. We found in human mouse cells, the deletion G3BP1 led to dampened...

The unique taste induced by monosodium glutamate is referred to as umami taste. The umami taste is also elicited by the purine nucleotides inosine 5'-monophosphate and guanosine 5'-monophosphate. There is evidence that a heterodimeric G protein-coupled receptor, which consists of the T1R1 (taste receptor type 1, member 1, Tas1r1) and the T1R3 (taste receptor type 1, member 3, Tas1r3) proteins, ...

Sperm-specific phospholipase C zeta (PLCζ) initiates intracellular calcium (Ca2+) transients which drive a series of concurrent events collectively termed oocyte activation. Numerous investigations have linked abrogation and absence/reduction PLCζ with forms male infertility in humans where activation fails. However, very few studies examined potential relationships between advancing age, both ...

background: leishmania major lmsti1 is a conserved protein among different species of leishmania, and expressed in both amastigote and promastigote forms of l. major life cycle. it has previously been expressed in bacterial systems. m aterials and methods: to express lmsti1 in the methylotrophic yeast         pichia pastoris ( p. pastoris ), the shuttle vector ppicza containing gene lmsti1 was ...

Humans navigate crowded spaces such as a university campus by following common sense rules based on social etiquette. In this paper, we argue that in order to enable the design of new target tracking or trajectory forecasting methods that can take full advantage of these rules, we need to have access to better data in the first place. To that end, we contribute a new large-scale dataset that co...

conclusions the results of this work demonstrated that the subcellular distribution of pfdn1 was altered in the rabv-infected n2a cells and colocalized with the n protein of rabv in the nbl structures. results confocal microscopy showed that pfdn1 was colocalized with the n protein of rabv in the infected n2a cells and was mainly recruited to the characteristic negri-body-like (nbl) structures ...

background il-33 is a novel member of the il-1 family, which has been shown to play an important role in t helper 2 (th2)-associated immune responses. recent studies have suggested a possible role for il-33 in the pathogenesis of liver damage during acute and chronic hepatitis; furthermore, il-33 may be involved in the development and progression of liver fibrosis. objectives to evaluate serum ...

Background: The production of recombinant proteins in Escherichia coli is one of the most valuable achievements in biotechnology, with many therapeutic and diagnostic applications; however, the aggregation and misfolding of proteins that result in the formation of insoluble inclusion bodies is a disruptive factor in this process. Various solubilization and refolding methods can be used to impro...

Bovine Viral Diarrhea virus (BVDV) is an important viral pathogen of cattle causing several clinical syndromes. There are usually no pathognomonic clinical signs of BVDV infection. Diagnostic investigations therefore rely on serological detection and virus isolation. Nonstructural protein 3 (NS3) as immunogenic protein of BVDV is genetically and antigenically conserved among different isolates....