An efficient regeneration protocol was developed from shoot tip and nodal explants of Simarouba glauca DC, a promising biodiesel plant. Nodal explants appeared to have better regeneration capacity than shoot tip explants (40%) in the tested media. The highest regeneration frequency (90%) and shoot number (7.00 ± 1.00 shoots per explants) were obtained in nodal explants in Murashige and Skoog’s ...

This paper describes attempts to localize the site of perception of low temperatures (0-10 degrees C) during thermoinduction in Thlaspi arvense L. Reproductive development (stem elongation and flower formation) was observed when shoots were cooled to 4 degrees C for 4 weeks and then returned to 21 degrees C while maintaining the roots constant 21 degrees C. However, chilling the roots was ineff...

Shoot multiplication of Bupleurum distichophyllum was achieved from the nodal and shoot tip explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response was from axillary shoots and the highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of axillary shoot proliferation was fou...

Explants of leaves, petals, anthers, styles and ovaries of C. speciosus, C. cancellatus, C. gilanicus and C. almehensis were cultured on LS media with three different combinations of NAA and BAP in the dark. Callus and neoformant organs (leaf and corm-like structure and ordinary and contractileroots) were fomd on some of these explants. Corm explants from these species were also cultured on...

In vitro preservation of the “Kober 5BB” rootstock (Vitis berlandieri ˆ Vitis riparia) was assessed with the encapsulation technique and slow growth storage. Shoot tips and nodal segments excised from in vitro cultures were encapsulated in calcium-alginate beads. A 30 min ion exchange time proved optimal for forming proper beads. The encapsulated and naked explants were stored at 4 ̋C in the da...

The seeds of ten different genotypes of chickpea (Cicer arietinum L.) originated from Egypt, ICARDA and Saudi Arabia, were used to screen their tissue culture response on various media combination. MS media supplemented with 1, 2 and 4 mg of each of IBA and transzeatin /l, were tested. The results showed that stem results were far better from leaves explants. The callus induction ranged from 6....

Explants of tobacco pith taken at various distances from the apex of a mature stem show a sharp gradient in growth potential in vitro; growth is highest in the extreme apical and basal explants, and is minimal in explants removed ca. 75 cm from the apex. Calluses produced by the vigorously growing basal explants are harder and more compact than those produced from more apical explants. The grad...

Micropropagation of three genotypes of Indian mustard [Brassica juncea (L.) Czern.] using 7-days old seedling-derived transverse thin cell layer (tTCL) explants was accomplished. The genotype, explant source and addition of silver nitrate to the medium significantly influenced shoot bud induction. MS medium with 26.6 μM of 6-Benzylaminopurin (BAP) and 3.22 μM of 1-naphtaleneacetic acid (NAA) wa...

A method for tissue culture of micropropagation aquatic plant Staurogyne repens (Nees) Kuntze has been developed. Different phytohormones were investigated to optimize the growth explants. The shoot tip explants S. placed in MS nutrient medium including BAP (0.30-1.50 mg/l) + TDZ (0.10 NAA and Kn mg/l). Shoot regeneration frequencies changed as 77.77-100% 66.66-100% cultivation augmented with B...

A novel protocol for indirect shoot organogenesis of Adhatoda vasica was developed using petiole explants derived from mature shrubby plants. Media with concentrations of cytokinins in combination with auxins were used to induce callus formation in two explants types: petiole and leaf segment. The frequency of callus formation from petiole and leaf segment explants on Murashige and Skoog (MS) b...