نتایج جستجو برای: shoot regeneration
تعداد نتایج: 77450 فیلتر نتایج به سال:
In this report we describe a methodology for rapid sugarcane shoot regeneration using meristematic tissue from in vitro-grown plantlets. Shoot regeneration from previously induced meristematic tissue, in the presence of up to 22.62 μM of 2,4-D was evaluated. The explants were transferred, after a week induction, to a propagation medium supplemented with activated charcoal and then transferred t...
a reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant vitex negundo through in vitro culture. of different concentrations of n6-benzyladenine (ba) evaluated as supplements to murahige and skoog (ms) medium, ba at 1mg/l was effective in enhancing bud break and shoot regeneration. response of explants was al...
Anthemis hyalina DC. is one of the most important herbal medicine plants and is used for anti-allergy and curing skin rashes or acne. To date there is no report for in vitro culture of this species and the present study was performed for the first time with the aim of obtaining the optimized treatment for shoot regeneration. In this research the effect of different plant growth regulators NAA (...
Salicornia europaea is a halophytic annual plant of the Chenopodiaceae family, which is considered as an oil plant in marine irrigation. This experiment was conducted to determine the effect of salinity and to reach the best hormonal treatment for shoot regeneration. After washing and sterilization, seeds of S. europaea were cultured under controlled light and temperature conditions at five sal...
Different concentrations of N(6)-benzylaminopurine (BAP) and indole acetic acid (IAA) in Murashige and Skoog based medium were assessed for their effects on shoot multiplication, nodule-like meristem proliferation and plant regeneration of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak. BAP at 1-14 mg L(-1) with or without 0.2 mg L(-1) IAA, or BAP at 7...
The human herpes simplex virus thymidine kinase type 1 gene (HSVtk) acts as a conditional lethal marker in mammalian cells. The HSVtk-encoded enzyme is able to phosphorylate certain nucleoside analogs (e.g. ganciclovir, an antiherpetic drug), thus converting them to toxic DNA replication inhibitors. The utility of HSVtk as a conditional negative-selection marker was explored in Arabidopsis thal...
l h e human herpes simplex virus thymidine kinase type 1 gene (HSVtk) acts as a conditional lethal marker in mammalian cells. The HSVtk-encoded enzyme is able to phosphorylate certain nucleoside analogs (e.g. ganciclovir, an antiherpetic drug), thus converting them to toxic DNA replication inhibitors. l h e utility of HSVtk as a conditional negative-selection marker was explored in Arabidopsis ...
In micropropagation of Anthurium the most important and sensitive stage, is callus induction from explant. This experiment was conducted in a completely randomized design with factorial arrangement in 4 replications in Sari Agriculture Sciences and Natural Resources University (SANRU) in 2010. The cultivar used in this study is Fire and leaf segments with and without main nervure, were used as ...
In vitro morphogenesis is greatly influenced by plant growth regulators (PGRs). The effect of different plant growth regulators was assessed on callus induction, in vitro shoot regeneration and hairy root formation in tomato. The maximum callus induction frequency was recorded culturing hypocotyls, while in vitro shoot regeneration frequency was significantly higher when leaf discs were used as...
Plant regeneration can occur via in vitro tissue culture through somatic embryogenesis or de novo shoot organogenesis. Transformation of soybean (Glycine max) is difficult, hence optimization of the transformation system for soybean regeneration is required. This study investigated ENHANCER OF SHOOT REGENERATION 1 (GmESR1), a soybean transcription factor that targets regeneration-associated gen...
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