The aim of the present investigation was to standardize in vitro shoot tip culture technique considering various aspects for direct plant formation two onion (Allium cepa L.) varieties ‘Indian’ and ‘Taherpuri’. About 1.5-2.2 mm tips were collected from bulbs sterilized with 0.1% HgCl2 duration. Then they cultured on MS medium supplemented hormonal concentrations different media composition prim...

Shoot tip and nodal segment explants of Holarrhena antidysenterica when cultured on MS medium containing BAP (1.0-3.0 mg/l) with NAA (0.2-1.0 mg/l) and BAP (1.0-3.0 mg/l) with Kn. (0.2-1.0 mg/l) produced multiple shoots. Maximum multiple shoots was found in MS medium supplemented with BAP (2.0 mg/l) and NAA (0.5 mg/l). Subculture on the same medium resulted in rapid shoot multiplication at an a...

Micropropagation of Asclepias curassavica L. a medicinal plant valued for its medicinal properties has been carried out using two different plant culture media, Murashige and Skoog (MS) and L2. L2 medium proved to be superior to MS medium in terms of shoot multiplication and shoot length. Nodal explants showed enhanced organogenic response than shoot tip explants. Basal nodal explants produced ...

fig trees are threatened by the attack of fig mosaic disease (fmd) on leaves and fruits caused by viruses of several genera. shoot-tip culture is a convenient method for viral sanitation. for this purpose, a reliable protocol for rapid in vitro propagation was developed with shoot-tips of three major tunisian local fig (ficus carica l.) varieties zidi (zdi), soltani (sni), bither abiadh (ba) an...

In order to select the best tomato cultivar for Agrobacterium mediated gene transformation studies, In vitro regeneration frequency of hypocotyls, leaf disc and shoot tip of five tomato cultivars (Lycopersicon esculentum) was investigated on a regeneration medium supplemented with 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. Significant differences in regeneration capacity between genotypes...