Homozygotes of the quail silver mutation, which have plumage color changes, also display a unique phenotype in the eye: during early embryonic development, the retinal pigment epithelium (RPE) spontaneously transdifferentiates into neural retinal tissue. Mitf is considered to be the responsible gene and to function similarly to the mouse microphthalmia mutation, and tissue interaction between R...

Coomassie Brilliant Blue (CBB) is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. Furthermore, it is completely compatible with mass spectrometric protein identification. But despite these advantages, CBB is regarded to be less sensitive than silver or fluorescence stainings and therefore rarely used for th...

Although copper-silver ionization (CSI) has been used for 30 years to inactivate Legionella bacteria and other opportunistic pathogens in water, the literature is a mix of both successes failures. This paper reviews technology case studies help improve success CSI installations. Important properly designed system capable consistent delivery copper/silver ions at their target levels. However, ev...

Cartilage cells are characterized by a high concentration of extracellular sulfated proteoglycan. Electron microscopic autoradiography was used to compare the incorporation of sulfate into proteoglycan by limb-bud chondrogenic and myogenic cells. From stage 19 to stage 21 there was no significant difference between the cartilage- and muscle-forming regions in the number of silver grains over ei...

Silver nanoplate hierarchical turreted ordered arrays were fabricated through an electro-deposition method on ordered acuate silicon nanocone templates. Such arrays can be used as SERS substrates for trace analyses of streptomycin sulphate, and exhibit high activity and stability. This work is of importance in practical applications based on the SERS effect of noble metal micro/nano-structured ...

ABSTRACT The fungus Pleospora papaveracea and Nep1, a phytotoxic protein from Fusarium oxysporum, were evaluated for their biocontrol potential on opium poppy (Papaver somniferum). Four treatments consisting of a control, P. papaveracea conidia, Nep1 (5 mug/ml), and P. papaveracea conidia plus Nep1 (5 mug/ml) were used in detached-leaf and whole-plant studies. Conidia of P. papaveracea remained...

We applied a simple sodium dodecyl sulfate-polyacrylamide gel electrophoresis method to urine. The method, developed for serum protein analysis (Clin Chem 1984;30:475-9), has a high sample throughput and gives excellent resolution with unconcentrated urine. It clearly distinguishes and characterizes proteinuric urine (7.5 microL) by Coomassie Blue staining and gives complex silver-stained patte...

The rates of 35S-sulfate incorporation into proteoglycan were compared in multi-scratch wounded and confluent cultures of bovine aortic endothelial cells to determine whether proteoglycan synthesis is altered as cells are stimulated to migrate and proliferate. Incorporation was found to be stimulated in a time-dependent manner, reaching maximal levels 44-50 h after wounding, as cells migrated i...