Single strand conformation polymorphism (SSCP) is a reproducible, rapid and quite simple method for the detection of deletions/insertions/rearrangements in polymerase chain reaction amplified DNA. All the details for the use of PCR-SSCP are presented in the direction of genetic diseases (beta-thalassaemia, cystic fibrosis), optimum gel conditions, sensitivity and the latest modifications of the...

The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect α-lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sa...

This study was conducted to investigate the association of Gonadotropin-Releasing Hormone Receptor (GnRHR) gene with growth traits in Awassi (n= 123) and Karakul 78) sheep. Starting from birth-day 12 months age, several were investigated, namely body weight length, wither rump height, chest, abdominal circumferences. Four fragments designed amplify three exons GnRHR gene, P1 P2 for exon 1, P3 2...

background: the incidence rate of breast cancer has been dramatically increasing since the last decade in iran, and it is now one of the most common female malignant tumors. b?cell lymphoma 2 (bcl2) family is the most important regulator of apoptosis, and ?938c>a single nucleotide polymorphism (snp) of bcl2 gene promoter has been demonstrated to influence breast cancer susceptibility. in this r...

genotypes of iranian zel sheep for calpastatin(cast) locus were determined by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) and polymerase chain reaction-single strand conformation polymorphism (pcr-sscp) methods and for calpain(capn) locus by pcr-sscp. blood samples were collected from 200 purebred zel sheep of zel breeding station located in golestan province i...

The purpose of this study was to assess and explain the relationship between molecular changes occurring in squamous cell laryngeal carcinoma. Furthermore, we were revealed the role of FHIT gene response in the development and progression of squamous cell laryngeal carcinoma, and genes based exons 7,8 and 9 in tumour stage and histopathologic grade. In this study was used 50 blood samples for c...

Background: Interleukie-8 is a proinflammatory cytokine and a potent chemotactic and activation factor for neutrophils. It has been reported to be expressed in high levels by various cell types after immune stimulation and mediates neutrophil recruitment in host. Objectives: The aim of this study was to analysis of the relationship between single nucleotide polymorphisms (SNPs) at position -18...