In the present investigation, antimutagenic effect of petroleum ether, chloroform, methanol and water extracts of green tea was evaluated in Salmonella typhimurium TA-98 and TA-100 strains. Well known mutagens like sodium azide and daunomycin were added at a concentration of 10µl and 6µl per plate respectively resulted in the induction of histidine revertant colonies. However addition of 10 µl ...

Two questions have arisen concerning the article of Smuckler and Appleman (Appl. Microbiol. 12:355, 1964). The question of the development of toxicity in sodium azide media had been studied in our laboratory, but these data were omitted from the publication as it was not necessarily relevant to the reported results. Gerencsen and Weaver (Appl. Microbiol. 7:113, 1959) reported that sodium azide ...

In a previous publication Bondi and Dietz (1947) reported that various factors could influence the susceptibility of penicillinase-producing bacteria to penicillin. The authors concluded that individual cells of certain organisms that produce this enzyme may be just as susceptible to penicillin as those of organisms that do not produce penicillinase. In a continuation of this study, the effect ...

An efficient and straightforward method for one-pot synthesis of aryl azides from the corresponding amines, employing tributylstannyl azide as transfer reagent of N3 group is described. In this procedure diazotization of aryl amines occurs under mild conditions using tert-butyl nitrite (t-BuONO) and p-toluenesulfonic acid (TsOH). A variety of substituted aryl amines, with both electron-withdraw...

SUMMARY Sodium azide, 2,4-dinitrophenol and iodoacetate did not inhibit hydrolysis of lactose by cell-free preparations of Sacchuromyces fragilis ,8-galactosidase, but with intact organisms fermentation and hydrolysis were inhibited to a similar extent. This suggests that these inhibitors may interfere with the transport of lactose into the cell. Galactose fermentation was inhibited by sodium a...

This paper reports the inhibition kinetics of mushroom (Agaricus bisporus; J.E. Lange) polyphenol oxidase (PPO) with benzoic acid, sodium azide and sodium fluoride as inhibitors, and pyrocatechol as substrate. Mushroom PPO was significantly inhibited by all of the tested inhibitors. Diphenolase activity of mushroom PPO exhibited the competitive-type inhibition with benzoic acid as an inhibitor;...

Due to the importance of amino acids (AAs) as basic bricks proteins and their application in drug food industries, there is great interest separation identification using simple inexpensive approaches. Application predictive models for determination behavior AAs can reduce trial-and-error experiments. Herein, retardation factor (RF) 21 protein were studied quantitative structureretardation (QSR...