The spectrophotometric determination of the catalase-H2O2 intermediate (Compound I) was extended to the liver in situ in anaesthetized rats. The rate of H2O2 production was determined for the liver in situ with endogenous substrates, and in the presence of excess of glycollate. Glycollate infusion doubled H2O2 production rate in the liver of air-breathing rats, and caused a fourfold increase wh...

Glycollate oxidase (glycollate:oxygen oxidoreductase, EC 1.1.3.1) from Pisum .f>ativum has an unusual absorption spectrum which suggests that the flavin N(3)-H function of the FMN coenzyme is ionized at pH 8.3. The enzyme is reduced rapidly by the substrate glycollate to yield a normal, reduced FMN coenzyme which is readily reoxidized by Oz. No evidence for the occurrence of covalent intermedia...

Euglena mitochondria contain both glyoxylate reductase (NADP+) and glycollate dehydrogenase to constitute the glycollate-glyoxylate cycle [Yokota & Kitaoka (1979) Biochem. J. 184, 189-192]. Euglena glyoxylate reductase (NADP+) was purified and its submitochondrial location was determined in order to elucidate the cycle. The purified glyoxylate reductase was homogeneous on polyacrylamide-gel ele...

Purpose: To optimize the process conditions and analyze in vitro digestibility of corn starch phosphodiester prepared by sodium trimetaphosphate (STMP). Methods: By using response surface method, the effects of STMP concentration, pH, esterification temperature, and urea addition on digestion resistance of corn starch phosphodiester were investigated and optimal conditions were determined. Corn...

Purpose: To analyze the physicochemical properties and in vitro digestibility of sweet potato starch phosphodiester prepared using sodium trimetaphosphate. Methods: The physicochemical properties of sweet potato starch phosphodiester were analyzed by using infrared spectrometry (IR), differential scanning calorimetry (DSC) and rapid visco-analyser (RVA). In addition, an in vitro digestibility m...

The aim of this study was to prepare fast-dissolving tablets of meloxicam after its complexation with β-cyclodextrin (β-CD) and to investigate the effect of using different superdisintegrants on the disintegration and release of meloxicam from the tablets. A complex of meloxicam with β-CD was prepared by spray drying and then compressed in the form of tablets utilizing the direct compression te...

In the present study, fast disintegrating tablets were designed with a view to enhance patient compliance. In this method, the hull of Hordeum vulgare, cross carmellose sodium, and sodium starch glycolate were used as superdisintegrants (4 and 6%), along with microcrystalline cellulose and mannitol, to enhance mouth feel. The prepared batches of tablets were evaluated for hardness, friability, ...

in the present study, floating drug delivery beads (fdds) were prepared with sodium alginate/ starch blend as a matrix, sodium hydrogen carbonate as a pore forming agent, methyl cellulose as a binder and barium chloride solution as a hardening agent. in order to prepare the beads with different porosity and morphology the ratio between pore forming agent to polymer blend and ratio of the consti...

Starchgelatin binary binder mixtures were applied in the formulation of sodium salicylate tablets. Locally developed cassava starch (tapioca starch) was used as a co-binder with gelatin. Wet granulation method was used to formulate different batches of sodium salicylate tablets with varying binder concentrations (1-5%w/w) and starch – gelatin binder ratios (25:75, 50:50, 75:25 and 0:100). The s...

Background: Although advantages of immobilization of cells through entrapment in calcium alginate gel beads have already been demonstrated, nevertheless, instability of the beads and the mass transfer limitations remain as the major challenges.Objective: The objective of the present study was to increase the stability, porosity (reduce mass transfer limit...