1. Solubility curves of crude pepsin preparations indicate the presence of more than one protein. 2. One of these proteins has been isolated and crystallized and found to have constant activity and constant solubility in several solvents. 3. The solubility measurements are complicated by the unstable nature of the protein and the fact that in certain solvents the solubility of the protein is ma...

We propose a new electrochemical method for the measurement of solubility product oxide metal impurity, in liquid solvent that is less easily oxidized than impurity. When known amount oxygen continuously added into by coulometric titration, dissolved impurity reacts with oxygen, forming oxide. By measuring concentration during can be derived. From this product, using thermochemical data chemica...

Methods for the purification and crystallization of tetanal toxin are described. The methods consist of the multiphase fractionation system involving methanol as the organic precipitating agent under controlled conditions of pH, ionic strength, protein concentration, and temperature. Crystalline tetanal toxin has an electrophoretic mobility of 2.8 x 10(-5) in veronal buffer of 0.1 ionic strengt...

The effect of ethanol addition to aqueous semi saturated NaCl solutions on the super saturation was studied. The amount of precipitated salt was found to increase as the percent of added ethanol increased. The rates of formation of precipitate were calculated and found to increase with decrease of the weighted average of the solvents mixture dielectric constant, , but levels out at  ≈ 50. The...

In 1937, Logan and Taylor (1) came to the conclusion “that the bone salts cannot precipitate spontaneously from the bone plasma unless the ion product is increased. Once formed, the bone salts cannot dissolve unless the concentrations of the ions composing them are decreased below the concentrations found in the blood plasma.” This conclusion was based upon some observations which seemed to Log...

In 1937, Logan and Taylor (1) came to the conclusion “that the bone salts cannot precipitate spontaneously from the bone plasma unless the ion product is increased. Once formed, the bone salts cannot dissolve unless the concentrations of the ions composing them are decreased below the concentrations found in the blood plasma.” This conclusion was based upon some observations which seemed to Log...

In 1937, Logan and Taylor (1) came to the conclusion “that the bone salts cannot precipitate spontaneously from the bone plasma unless the ion product is increased. Once formed, the bone salts cannot dissolve unless the concentrations of the ions composing them are decreased below the concentrations found in the blood plasma.” This conclusion was based upon some observations which seemed to Log...

Oligophenylalanines are soluble in m-cresol, but oligophenylalanyl-tRNAs are not. This differential solubility can be used to assay oligophenylalanines released from tRNA during their synthesis by wheat germ extracts. When poly U is the message, virtually no free product appears. When poly A U (A < U) is used, a considerable amount of oligophenylalanines are released. The fraction of product re...