The udhA gene of Escherichia coli was cloned and expressed in E. coli and found to encode an enzyme with soluble pyridine nucleotide transhydrogenase activity. The N-terminal end of the enzyme contains the fingerprint motif of a dinucleotide binding domain, not present in published E. coli genome sequences due to a sequencing error. E. coli is hereby the first organism reported to possess both ...

There is a large collection of e ective algorithms for computing information about nite soluble groups. The success in computation with these groups is primarily due to a computationally convenient representation of them by means of (special forms of) power conjugate presentations. A notable omission from this collection of algorithms is an e ective algorithm for computing the automorphism grou...

The metabolism and macromolecular binding of the carcin ogen benzo(a)pyrene[B(a)P]and its relativelyinert structural isomer benzo(e)pyrene [B(e)P1 have been studied in order to determine if a metabolic basis exists for their very different biological activities. B(a)P and B(e)P are metabolized by hamster embryo cells to organic solvent-soluble and water-soluble metabolites. Signifi cant quantit...

Tuberculosis is characterized by the presence of activated mononuclear cells both in the peripheral circulation and in pleural fluid. Expression and up-regulation of adhesion molecules is the basis of cell-cell adhesion in granuloma formation and in leukocyte migration to the inflammatory site. Soluble isoforms of adhesion molecules have been described, and their expression at high levels indic...

Polymer blends of cold water soluble starches (amylose or amylopectin soluble starch) with gelatin were prepared using solvent casting method. The solid state miscibility and polymer-polymer interactions between the constituent polymers were studied by fourier transforms infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorirmetry (DSC), light optical microscopy (OP...

The metabolism and macromolecular binding of the carcin ogen benzo(a)pyrene[B(a)P]and its relativelyinert structural isomer benzo(e)pyrene [B(e)P1 have been studied in order to determine if a metabolic basis exists for their very different biological activities. B(a)P and B(e)P are metabolized by hamster embryo cells to organic solvent-soluble and water-soluble metabolites. Signifi cant quantit...

BACKGROUND The receptor for advanced glycation end-products (RAGE) has been suggested to modulate lung injury in models of acute pulmonary inflammation. To study this further, model systems utilizing wild type and RAGE knockout (KO) mice were used to determine the role of RAGE signaling in lipopolysaccharide (LPS) and E. coli induced acute pulmonary inflammation. The effect of intraperitoneal (...

BACKGROUND Gastric cancer remains a major cause of cancer mortality globally but no good prognostic tumour marker is available. Soluble fragment of E-cadherin protein has been reported to increase in the sera of patients with cancer and recently was found to be elevated in 67% of patients with gastric cancer. AIMS To investigate if serum soluble E-cadherin is a valid prognostic marker in gast...

background: recombinant proteins overexpressed in e. coli are usually deposited in inclusion bodies. cysteines in the protein contribute to this process. inter- and intra- molecular disulfide bonds in chitinase, a cysteine-rich protein, cause aggregation when the recombinant protein is overexpressed in e. coli. hence, aggregated proteins should be solubilized and allowed to refold to obtain nat...