The method is based on the selective activating capacity of dithiothreitol on creatine kinase isoenzyme MB, after isoenzyme MM is activated by glutathione. Isolated isoenzymes MM and MB of human and canine origin were assayed individually and in mixtures of known activities. When glutathione was present in the assay medium the activity of each isoenzyme could be measured individually, but gluta...

The aim of the study was to evaluate usefulness determining markers myocardial damage, such as: aspartate aminotransferase, total creatine kinase, cardiac kinase isoenzyme, lactate dehydrogenase, dehydrogenase isoenzyme and peripheral blood lactic acid in sport horses subjected physical exertion. research carried out on 50 three breeds: Ukrainian Riding, Hanoverian Westphalian, aged 4-16 years....

In this study, we used Random Amplified Polymorphic DNA (RAPD) for identification of 17 isolates of Leishmania from the skin and reticuloendothelial system of humans, animal reservoirs (rodent and dog) and sandflies in various parts of Iran in the last decade. Fifteen species have been confirmed by isoenzyme characterization by the London School of Hygiene and Tropical Medicine and Shiraz ...

The diagnosis of amebiasis by microscopic identification of the parasite in stool is insensitive and unable to distinguish the invasive parasite Entamoeba histolytica from the commensal parasite E. dispar. In this study, we have tested a PCR technique for the detection of E. histolytica and compared it with isoenzyme analysis and the TechLab E. histolytica-specific antigen detection test. The n...

The total lactic dehydrogenase (LD) content and LD isoenzyme ratios were studied in homogenized rectal mucosa from 31 patients with established non-specific mucosal ulcerative colitis and from 16 normal subjects. The total LD content was found to be significantly increased in patients with active ulcerative colitis when compared with patients with inactive colitis or with normal subjects. There...

To purify cytoplasmic and mitochondrial isoenzymes of aspartate aminotransferase (EC 2.6.1.1) from human liver. We used heat treatment, ammonium sulfate precipitation, anion- and cation-exchange chromatography, affinity chromatography, and isoelectric focusing. Final preparations of the isoenzymes were homogeneous, with specific activities of 198 and 208 kU/g for the cytoplasmic and the mitocho...

We examined the stability of human lactate dehydrogenase (EC 1.1.1.27) isoenzyme 5--purified to a specific activity of about 400 kU/g--when lyophilized in a buffered, stabilized matrix of bovine albumin. This isoenzyme was prepared with a final activity of about 500 U/L and stored at -20, 4, 20, 37, and 56 degrees C for as long as six months. This isoenzyme decayed with approximate first-order ...

Because previous reports have given inconsistent results, we re-examined the catalytic concentrations of cytoplasmic creatine kinase (CK) and of CK isoenzymes in 38 biopsies obtained from 19 different tissues. After homogenization and centrifugation many tissues showed high CK catalytic concentrations; 11 of them contained activity exceeding 50 U/g wet weight (Scandinavian recommended method). ...

1. GM,-ganglioside, specifically tritiated in the terminal galactose, was hydrolysed by two forms of 'acid' methylumbelliferyl fl-galactosidase isolated on gel filtration. 2. Identification of GM1-ganglioside ,B-galactosidase activity with the 'acid' methylumbelliferyl ,-galactosidases was based on the following: coincident elution profiles on gel filtration; simultaneous inactivation by heat a...