We developed a continuous spectrofluorimetric assay of lysoplasmalogenase activity with the use of horse liver alcohol dehydrogenase as a coupling enzyme. In this method the disappearance of NADH is measured spectrofluorimetrically. The excitation and emission monochromators were set at 340 and 460 nm respectively. The assay is 10 times as sensitive as the previous u.v. spectrophotometric metho...

A simple spectrofluorimetric analysis of a local anaesthetic named procaine using a specific labelling reagent for primary amino groups, has been developed. Because procaine shows very weak native fluorescence, the technique of spectrofluorimetry has been very much limited for its determination. A detail study of the variables affecting the derivatisation reaction (pH, fluorescamine (FC) concen...

Gabapentin is an anticonvulsant widely used in the treatment of epilepsy.A simple, rapid, accurate, precise and economic spectrofluorimetric methods have been developed for the estimation of gabapentin in pharmaceutical formulations and in spiked human plasma. The analysis and validation dine by using SHIMADZU RF – 5301 Spectofluorimeter, equipped with Xenon arc lamp, using quartz cell (1×1×4.5...

Simple and sensitive spectrofluorimetric method for phosphorus determination in food samples after microwave mineralisation has been described. The proposed method is based on the formation of a fluorescent chelate between morin and aluminium and the fluorescence quenching after addition of phosphates solution. Additionally, the method with quinine sulphate was modified and applied for food sam...

The development of the spectrofluorimetric method can be considered a promising alternative that is relatively less expensive and sufficiently reliable. In current literature, no for analysis nevirapine by spectro-fluorimetric has been reported. proposed based on transformation naturally non-fluorescent into fluorescent derivative after chemical synthesis. Maximum excitation emission wavelength...

A library of azonia aromatic cations has been studied in order to gain insights into the effect of the size, shape and charge distribution on the fluorescence, DNA interactions and DNA sequence selectivity properties. Fluorescence-based thermal denaturation experiments, spectrofluorimetric titrations, circular dichroism measurements and theoretical simulations have shown that some of the studie...

Bacterial resistance to antibiotics due increased efficiency of the efflux is a serious problem in clinics infectious diseases. Knowledge factors affecting activity pumps would help find solution. For this, fast and trustful methods for analysis are needed. Here, we analyzed how assay conditions affect accumulation indicators ethidium (Et+) tetraphenylphosphonium Salmonella enterica ser. Typhim...