نتایج جستجو برای: spectrophotometric assay
تعداد نتایج: 232690 فیلتر نتایج به سال:
A continuous spectrophotometric assay has been developed for detecting β-glucuronidase activity. In the assay, Para-nitrophenyl β-D-glucuronide is cleaved to yield a chromophoric product. With the commercial E. coli enzyme, it is demonstrated that the reactions can be continuously monitored by the increase of absorbance at 405 nm. The method is highly sensitive and able to detect less than 1.4 ...
Famotidine belongs to H2-receptor antagonist. It is used widely for the treatment of treatment of (GERD) gastro-esophageal reflux disease and gastric ulceration duodenal ulcer, stress ulcer. A simple, efficient spectrophotometric method for the assay of famotidine has been developed for estimation of famotidine formulations. Comparision of three different brands of famotidine (nocid, famulcer a...
A simple spectrophotometric assay for arogenate dehydratase, the enzyme that catalyzes the formation of L-phenylalanine from L-arogenate, is presented. The method couples the arogenate dehydratase reaction with that of an aromatic aminotransferase partially purified from Acinetobacter calcoaceticus. In the presence of 2-ketoglutarate, phenylpyruvate formation is measured at 320 nm at basic pH. ...
BACKGROUND Analysis of the pyruvate dehydrogenase complex (PDHc) activity in human skin fibroblasts is hampered by low enzyme activity in the cells. The most commonly used radiochemical method detects the formation of (14)CO(2), an endproduct of the E1 component of PDHc, from [1-(14)C]pyruvate. METHODS We report a spectrophotometric method for the analysis of PDHc activity in fibroblasts base...
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