Poly (2-hydroxyethyl methacrylate) (HEMA) has been used as a clinical material, in the form of a soft hydrogel, for various surgical procedures, including endovascular surgery of liver. It is a clear liquid compound and, as a soft, flexible, water-absorbing material, has been used to make soft contact lenses from small, concave, spinning molds. Primary rat hepatocyte spheroids were created on a...

In vitro models continuously evolve to more closely mimic and predict biological responses of living organisms. Just in the past years many novel three dimensional (3D) organotypic models, which resemble tissue structure, function and even disease progression, have been developed. However, application of more complex models and technologies may increase the risk of compromising assay robustness...

A technique has been developed which takes advantage of the spherical symmetry of EMT6/Ro multicellular tumor spheroids to isolate subpopulations of cells originating from various locations in the spheroid structure. The method involves gently exposing spheroids to a dilute trypsin solution at 18 to 20 degrees in specially designed dishes on a rotary shaker. Under these conditions, cells are re...

Breast cancer spheroids have been widely used as in vitro models of cancer stem cells (CSCs), yet little is known about their phenotypic characteristics and microRNAs (miRNAs) expression profiles. The objectives of this research were to evaluate the phenotypic characteristics of MDA-MB-231 spheroid-enriched cells for their CSCs properties and also to determine their miRNAs expression profile. S...

As part of a global analysis of deep star counts to constrain scenarii of galaxy formation and evolution, we investigate possible links between the galactic spheroid and the dark matter halo. A wide set of deep star counts at high and intermediate galactic latitudes is used to determine the large scale density law of the spheroid. Assuming a power density law, the exponent, flattening, local de...

We previously reported the development of a simple, user-friendly, and versatile 384 hanging drop array plate for 3D spheroid culture and the importance of utilizing 3D cellular models in anti-cancer drug sensitivity testing. The 384 hanging drop array plate allows for high-throughput capabilities and offers significant improvements over existing 3D spheroid culture methods. To allow for practi...

Various spheroid formation techniques have been widely developed for efficient and reliable 3-D cell culture research. Although those efforts improved many aspects of spheroid generation, the procedures became complex and also required unusual laboratory equipment. Many recent techniques still involve laborious pipetting steps for spheroid manipulation such as collection, distribution and resee...

The echo statistics associated with a randomly oriented prolate spheroid randomly located in the sonar beam are modeled. The cases of a smooth and rough spheroid, both in the water column (i.e., case in which boundaries are not involved), and on a boundary are investigated. The modeling begins with the analytical physics-based expression for the scattering amplitude of a smooth prolate spheroid...

We describe a program of star counts in the range 19 ∼< I ∼< 26 made with the WFPC cameras aboard the Hubble Space Telescope. Red (V − I > 1.0) stars at these magnitudes are primarily disk and spheroid M dwarfs. The stars are found both on dedicated images as part of the parallel program and by using appropriate archive data. We measure the faint end of the luminosity functions of the disk and ...

The effect of an intravenous injection of squid-ink (sepia-melanin) solution on adult mouse spheroid alveolar epithelial cells was observed by the electron microscope. Sepia-melanin particles were seen in all alveolar wall cells examined that seems to suggest the entrance of sepia-melanin particles into the spheroid alveolar epithlial cells from the alveolar blood capillary. In cases of large p...