AIMS To adapt an immunomagnetic capture (IMC) technique to concentrate and cultivate Mycobacterium bovis from environmental samples including soil, faeces and urine. METHODS AND RESULTS Cells of Myco. bovis BCG and wild-type Myco. bovis were successfully isolated and cultured from seeded and naturally infected materials respectively. The IMC cell recovery estimated by colony forming units (CF...

a capillary electrophoresis method was used for assay of some degradation products of carvedilol. the optimized parameters were as; running buffer 80 mm acetate dissolved in methanol/ethanol mixture (65:35 v/v), applied voltage of 19 kv, temperature is 20 ºc and the wavelength range of 200-350 nm. the results indicate that the proposed ce method could effectively separate carvedilol from its de...

Background: Studies with longitudinal and/or time-series designs would benefit from non-invasive methods to assess immune response. Immune response levels typically are measured with blood obtained by venipuncture, which is distressing to subjects and, thus, may adversely affect recruitment and retention of subjects in studies with multiple or repeated biological sampling. Aims: The study aims ...

Cucumber is one of the main vegetables in Iranian food basket. A wide range of pesticides are used for crops protection during the cultivation of vegetables such as cucumber due to heavy pest infestation. Analysis of pesticide residues in food and other environmental commodities have become essential requirement for consumers, producers, and food quality control authorities. This study was aime...

The concentrations of amoxicillin in pig plasma were determined by HPLC technique. Separation was achieved on a Lichrocart 250 x 4 mm Purospher column and detection was performed with a fluorescence detector. The mobile phase consisted of a mixture of acetonitrile with buffer solution (0.05 M KH2PO4, pH 5.6) delivered by a isocratic programme. For amoxicilin assay, the plasma samples were expos...

A simple determination method of diphenyl (DP) and o-phenylphenol (OPP) in agricultural products by GC/MS was examined. DP and OPP were extracted with ethyl acetate in the presence of anh. sodium sulfate. After addition of n-butanol, the extract solution was concentrated. Clean-up was achieved by shaking with graphitized bulk carbon (Supelclean ENVI-Carb). Addition of polyethylene glycol sharpe...

A high-performance reversed-phase liquid chromatographic method for quantification of metoprolol tartrate (MT) in human plasma is presented. A C18 column was used with acetonitrile–water–triethylamine 18:81:1 (v/v) as mobile phase and pinacidil monohydrate as internal standard (IS). UV detection was at 275 nm and MT and the IS were detected at retention times of 1.5 and 2.6 min, respectively. T...

A method is described for the semi-automated measurement of inulin concentrations in serum and urine in the presence of glucose. The concentration of glucose is measured simultaneously and is used to correct for almost all "inulin-like" interferences. The inulin standard curve is linear over a wide range (5-2500 mg/l). Between-run precision is < 6% and recovery from spiked sera is 98%. This met...

A reliable, rapid and accurate method based on spiked calibration curves and direct sample introduction was developed for determination of 17 pesticide residues in rice by gas chromatography-mass spectrometry single quadrupole selected ion monitoring GC/MS-SQ-SIM. Sample preparation is based on extraction with acetonitrile without clean up. The use of spiked calibration curves for constructing...