Objective(s): The use of antisense oligonucleotides (AOs) to restore normal splicing by blocking the recognition of aberrant splice sites by the spliceosome represents an innovative means of potentially controlling certain inherited disorders affected by aberrant splicing. Selection of the appropriate target site is essential in the success of an AO therapy. In this study, in search for a splic...

Introduction: Pathogenic variants of RUNX2, a gene that encodes an osteoblast-specific transcription factor, have been shown as the cause of CCD, which is a rare hereditary skeletal and dental disorder with dominant mode of inheritance and a broad range of clinical variability. Due to the relative lack of clinical complications resulting in CCD, the medical diagnosis of this disorder is challen...

Abstract This paper describes the bond strength comparison between concrete and reinforcement with several types of concrete. The studied were normal (NC), Self-Compacting Concrete (SCC), High-Volume Fly Ash (HVFAC), (HVFA-SCC). Research data obtained from previous studies. by testing reinforced beam a lap splice in tensile moment area. Bond values normalized dividing square root grade. Then us...

Many of the mechanisms that govern splice site selection and splice site partner assignment during pre-mRNA splicing are obscure. To address this problem, we analyzed the splicing of transcripts containing chimeric introns or splice site duplications derived from two natural yeast genes. Our experiments indicate that there are strong context effects that influence splicing efficiency and relati...

the effect of a distributed patch mass on the natural frequency of vibration of a laminated rectangular plate with simply supported boundaries is investigated. the third order displacement field of a composite laminated rectangular plate is defined using the two variable refined plate theory. equations of motion of the plate are obtained with the help of calculus of variation. parametric study ...

Alternative 5' splice site selection is one of the major pathways resulting in mRNA diversification. Regulation of this type of alternative splicing depends on the presence of regulatory elements that activate or repress the use of competing splice sites, usually leading to the preferential use of the proximal splice site. However, the mechanisms involved in proximal splice site selection and t...

Recently we characterized three distinct GnRH receptors in the bullfrog (bfGnRHR-1, bfGnRHR-2, and bfGnRHR-3). In the present study, we further investigated the expression and function of splice variants, generated from the primary bfGnRHR-3 transcript by exon skipping (splice variant 1), intron retention (splice variants 2 and 3), and/or transcriptional slippage (splice variant 4), apart from ...

The transcription of the HIV-1 provirus results in only one type transcript—full length genomic RNA. To make mRNA transcripts for accessory proteins Tat and Rev, RNA must completely splice. Vif, Vpr, Env undergo splicing but not completely. Genomic (which also functions as Gag Gag/Pro/Pol precursor polyproteins) splice at all. can tolerate a surprising range relative abundance individual transc...

Large alternatively spliced internal exons are uncommon in vertebrate genes, and the mechanisms governing their usage are unknown. In this report, we examined alternative splicing of a 1-kb internal exon from the human caldesmon gene containing two regulated 5' splice sites that are 687 nucleotides apart. In cell lines normally splicing caldesmon RNA via utilization of the exon-internal 5' spli...

The fourth exon of the mouse polymeric immuno-globulin receptor (pIgR) is 654 nt long and, despite being surrounded by large introns, is constitutively spliced into the mRNA. Deletion of an 84 nt sequence from this exon strongly activated both cryptic 5' and 3' splice sites surrounding a 78 nt cryptic intron. The 84 nt deletion is just upstream of the cryptic 3' splice site; the cryptic 3' spli...