The potential cytotoxic activity of purified staphylococcal enterotoxins for mammalian cells was evaluated. The effects of staphylococcal enterotoxins A (SEA) and B (SEB) on cell membrane integrity as measured by leakage of labeled cytoplasmic constituents ([3H]uridine), amino acid transport (lysine and aminoisobutyric acid), and macromolecular synthesis (protein, ribonucleic acid, and deoxyrib...

Staphylococcus aureus produces a variety of toxins, enzymes, and metabolites, among which are enterotoxins A and B, exotoxins associated with certain outbreaks of food poisoning (2). Recent reports have established the lethal nature of enterotoxin B after its intravenous injection, with death in experimental animals characterized by profound shock (3-5). Fever and several other toxic manifestat...

Up to approximately 50 mug of staphylococcal enterotoxin A per plate was produced by cellophane-over-agar cultures. Enterotoxin was determined by a latex agglutination technique.

The cross-reactivity of monoclonal antibodies produced against staphylococcal enterotoxin A with purified and crude enterotoxins B, C1, D, and E and the specificity of such reactions were evaluated by the indirect enzyme-linked immunosorbent assay and immunoblotting of Western blots (from sodium dodecyl sulfate-polyacrylamide gel electrophoresis) followed by autoradiography. Purified and crude ...

A simple homogeneous enzyme immune assay was developed for detection of staphylococcal enterotoxin B by a conjugate of beta-amylase coupled with the enterotoxin.

Three variants of the chloramine-T radioiodination method were used to iodinate staphylococcal enterotoxin A with 125I. Only one method consistently produced usable labels for radioimmunoassay. The iodine incorporation was 55 to 76%; the specific activity was 3.5 to 5.5 muCi/microgram of enterotoxin, and the label was extremely stable on storage at -20 degrees C. Determinations of the enterotox...

Staphylococcus aureus is an important opportunist that can cause superficial to life-threatening illnesses in humans and a variety of animal species. Staphylococcal enterotoxin has an important role in food poisoning in human. Staphylococcal enterotoxin A is the most commonly reported enterotoxin in staphylococcal food poisoning. This study revealed the presence of enterotoxin (like) genes (sea...