BACKGROUND Protein crystallisation screening involves the parallel testing of large numbers of candidate conditions with the aim of identifying conditions suitable as a starting point for the production of diffraction quality crystals. Generally, condition screening is performed in 96-well plates. While previous studies have examined the effects of protein construct, protein purity, or crystall...

Detecting periodicity signals from time-series microarray data is commonly used to facilitate the understanding of the critical roles and underlying mechanisms of regulatory transcriptomes. However, time-series microarray data are noisy. How the temporal data structure affects the performance of periodicity detection has remained elusive. We present a novel method based on empirical mode decomp...

A peptide that functions only in the presence of a protein has been developed using reaction-based selection from peptide phage libraries. The peptide was not functional in the absence of the protein, but formed enaminones with 1,3-diketone derivatives when bound to the protein.

Of 700 coagulase-positive staphylococcal strains isolated from humans, 692 (98.9%) produced protein A. Of 100 coagulase-negative strains, 2 were protein A-positive.

How to recognize the structural fold of a protein is one of the challenges in protein structure prediction. We have developed a series of single (non-consensus) methods (SPARKS, SP(2), SP(3), SP(4)) that are based on weighted matching of two to four sequence and structure-based profiles. There is a robust improvement of the accuracy and sensitivity of fold recognition as the number of matching ...

Although a number of scientific advances have been made in the area of structural biology, a few obstacles continue to impede our ability to quickly and efficiently characterize protein structure-function relationships. Probability Density Profile Analysis (PDPA) is a method which rapidly quantifies the structural novelty of a protein, based on the statistical analyses of a minimal amount of em...

A system consisting of a protein LG coated surface for the capture of mammalian antibodies (target), and an antigen fused to Tus and stoichiometrically linked to a DNA template via the Tus-Ter-lock sequence allowed the ultrasensitive detection of 5.5 attomol of target by real-time immunoPCR in complex media.

The N-end rule relates the in vivo half-life of a protein to the identity of its N-terminal residue. Similar but distinct versions of the N-end rule operate in all organisms examined, from mammals to fungi and bacteria. In eukaryotes, the N-end rule pathway is a part of the ubiquitin system. I discuss the mechanisms and functions of this pathway, and consider its applications.

A total of 98.7% of streptococci, groups A,B,C, and G, isolated from various sources was correctly identified by the co-agglutination technique. The active components in this technique are protein A-containing staphylococci coated with antibodies specific for group A,B,C, and G streptococci. A suspension of streptococci belonging to one of these four groups co-agglutinates with the antibody-sen...