A single-copy reporter system for Staphylococcus xylosus has been developed, that uses a promoterless version of the endogenous beta-galactosidase gene lacH as a reporter gene and that allows integration of promoters cloned in front of lacH into the lactose utilization gene cluster by homologous recombination. The system was applied to analyze carbon catabolite repression of S. xylosus promoter...

A total of 221 strains of staphylococci and 98 strains of micrococci isolated from the skins of Eastern gray squirrels, Southern flying squirrels, raccoons, opossums, squirrel monkeys, swine, sheep, horses, cattle, and dogs were characterized in a preliminary attempt to resolve their natural relationships and distribution in nature. Staphylococci demonstrating the widest host range included Sta...

The study was aimed at characterizing the different Coagulasenegative staphylococci (CoNS) isolated from chronic skin ulcer out-patients attending four different hospitals in Kaduna State, Nigeria. A total of 292 swab samples were collected from January 2012 to January 2013. The samples were cultured using Chocolate and Blood agar media. Phenotypic characterization of the CoNS revealed the pres...

Based on structural and functional properties, three groups of large staphylococcal multiresistance plasmids have been recognized, viz., the pSK1 family, pSK41-like conjugative plasmids, and beta-lactamase-heavy-metal resistance plasmids. Here we describe an analysis of the replication functions of a representative of each of these plasmid groups. The replication initiation genes from the Staph...

An accurate evaluation of the seasonal dynamics of respiratory microbial flora in extensively raised goats represents the first step in early identification of potentially highly pathogenic bacteria in this species. The aim of the study was to monitor the seasonal influence on the bacterial flora of the animals, and therefore a comparative evaluation of changes during winter and spring seasons....