2.1.1 Aqueous extracts preparation The dried form of plant was mixed with sterile distilled water in a ration of 1:20 (100 g in 1 L solvent), and was stirred and heated for about 4 hours. After cooling, the extract was filtered by using Whatman No.1 filter paper. The filtrate was collected and frozen in ice cube container. The frozen ice cube was freeze-dried (i.e. lyophilisation) to obtain con...

A preliminary paper (1) reported that a serologically reactive polysaccharide of dextran nature is produced from sucrose through the action of an enzyme or some similar heat-labile agent contained in sterile filtered extracts of Leuconostoc mesenteroides. The object of the present paper is to describe the preparation of the sterile extracts and to compare the chemical and serological properties...

Other measures will also help to reduce the risk of postoperative endophthalmitis: proper hand washing (see ‘how to’ article on page 17), the use of sterile instruments, the ‘non-touch’ technique, the subconjunctival injection of antibiotics,4,5 and the intracameral injection of cefuroxime6 at the end of surgery (see article on page 11). The dose of intracameral cefuroxime must be meticulously ...

A new method of inoculation of gladiolus with cucumber mosaic virus (CMV) was developed using the Bio-Rad Helios Gene Gun System. This method circumvents the traditional use of aphids to transmit CMV, a virus that is mechanically transmissible to many plant species but only with difficulty to gladiolus. Cartridges containing virus-coated gold microcarriers were prepared and the virus shot into ...

A prerequisite for the prolonged infusion of a drug via a totally implanted drug delivery system is that the drug solution must be sufficiently stable at physiological temperatures to endure the time intervals between drug replacement or pump refills. Consequently, the stability of the chemotherapeutic agents can influence the dosing accuracy and ultimately the achievement of the desired therap...

only DESCRIPTION Coagulation Factor IX (Human), Mononine, is a sterile, stable, lyophilized concentrate of Factor IX prepared from pooled human plasma and is intended for use in therapy of Factor IX deficiency, known as Hemophilia B or Christmas disease. Mononine is purified of extraneous plasma-derived proteins, including Factors II, VII and X, by use of immunoaffinity chromatography. A murine...

Methods The introduction of a sequence of interventions targeted at reducing MRSA bacteraemias by a newly expended infection control team, after analysis of existing polices and procedures. The use of MRSA antimicrobials in the Trust’s formulary was optimised. Teicoplanin was substituted with daptomycin in medical and surgical wards. In Intensive Care vancomycin by continuous infusion was intro...

AIM To evaluate the efficacy of 0.5%, 2.5% and 5.25% sodium hypochlorite (NaOCl) as intracanal irrigants associated with hand and rotary instrumentation techniques against Enterococcus faecalis within root canals and dentinal tubules. METHODOLOGY A total of 180 extracted human premolar teeth were infected for 21 days with E. faecalis. The specimens were divided into 12 groups, as follows: gro...