The pathways of adrenal and ovarian steroid biosynthesis use the same enzymes for the initial steps of steroidogenesis but express different enzymes that convert steroid precursors to the final active products. Both the adrenal and ovary produce dehydroepiandrosterone (DHEA), the principal precursor of androgens and estrogens. The key enzyme in DHEA production is P450c17, which catalyzes both 1...

The pathways of adrenal and ovarian steroid biosynthesis use the same enzymes for the initial steps of steroidogenesis but express different enzymes that convert steroid precursors to the final active products. Both the adrenal and ovary produce dehydroepiandrosterone (DHEA), the principal precursor of androgens and estrogens. The key enzyme in DHEA production is P450c17, which catalyzes both 1...

The only practical means of maintaining a raised output of steroids from the adrenal cortex is by the daily intramuscular or subcutaneous injection of corticotropin in a medium that delays absorption. The effects produced by such therapy depend upon -the amount by which the daily output of steroids is raised above the starting level and the length of time that the raised level is maintained. Th...

Numerous methods are available for measuring urinary steroids in evaluating endocrine dysfunctions. Measurements of these particular steroids, or groups of them, usually involve tedious isolation methods, corrections for interferences and losses of the steroid, and (or) expensive reagents. We show how gas-liquid chromatography provides a rapid, sensitive, and direct method for several steroid m...

The aim of this study was to evaluate the concentrations of reproductive hormones in blood plasma and in follicular fluids of different sized follicles during various stages of the estrous cycle in Holstein cattle. Jugular blood samples from 42 adult Holstein cattle were collected immediately after the slaughter. Antral fluids from small (4-5 mm), medium (6-9 mm) and large (10-20 mm) follicles ...

s of presentations given in session 8 of the 2008 UCOWR Conference. This Article is brought to you for free and open access by the Conference Proceedings at OpenSIUC. It has been accepted for inclusion in 2008 by an authorized administrator of OpenSIUC. For more information, please contact [email protected]. Recommended Citation Deksissa, Tolessa, "Fate and Transport of Steroid Hormones in t...

The present work was initiated by the observation of Reifenstein, Homburger & Dobriner (1950) that 3a: 1 1l-dihydroxy-5p-androstan-17-one (1 l,B-hydroxyaetiocholanolone), a minor component of the urinary 17-oxo steroids, was significantly associated with neoplastic growth. The re-examination of this problem has led to the development of a micro method for the quantitative separation and determi...

J. TECHNIC was (lescribe(l by Few (1) in 1961 for deterniiiiation of I 7-hydroxycorticosteroids in which borohydride re(luction of steroid 20-keto groups was followed by oxidation of adjacent hydroxyl groups in the 17-20 positions with nletaperio(late. The borohydride also reduced all preformed 17-ketosteroids to the corresponding alcoholswhich do not give the Zimmerman reaction-alici reduced s...

the aim of this study was to evaluate the concentrations of reproductive hormones in blood plasma and in follicular fluids of different sized follicles during various stages of the estrous cycle in holstein cattle. jugular blood samples from 42 adult holstein cattle were collected immediately after the slaughter. antral fluids from small (4-5 mm), medium (6-9 mm) and large (10-20 mm) follicles ...

Treatment failure occurs in up to 30% of patients treated with steroids for inflammatory diseases. The aim of this study was to explore the potential role of 21 cytokines in steroid-resistant inflammatory disease and to develop methods to restore steroid sensitivity through cytokine manipulation. The dexamethasone inhibition of lymphocyte proliferation assay correlates with the outcome of stero...