abstractbackground: fetal bovine serum (fbs) is widely used in cell culture laboratories,risk of zoonotic infections and allergic side effects create obstacles for its use inclinical trials. therefore, an alternative supplement with proper inherent growthpromotingactivities is demanded.objective: to find fbs substitute, we tested human umbilical cord blood serum(hucs) for proliferation of human...

Background Endometriosis is the presence of endometrial tissue outside of the uterine cavity and is the most common gynecologic disorder in women of reproductive age. We have preliminary evidence that in the presence of a 3-dimensional (3-D) fibrin matrix, human endometrial glands, stroma, and neovascularization can develop in vitro, mimicking the earliest stages of endometriosis. The aim of th...

IgA vasculitis (IgAV) is a systemic, immune complex–mediated, small-vessel leukocytoclastic vasculitis. In the cutaneous tissue, it characterized by nonthrombocytopenic palpable purpura. Increasing evidence suggests importance of host immunity in its contribution to IgAV. However, molecular mechanisms tissue damage IgAV remain largely unknown. We performed single-cell RNA sequencing on biopsied...

GASTRO-INTESTINAL STROMAL-TUMOURs (GASTRO-INTESTINAL STROMAL-TUMOURs) are tumours of the gut found mostly in stomach and small intestine. The complications Gastrointestinal (GI) bleeding, obstruction, pain rarely perforation. We reporting an abnormal presentation STROMAL-TUMOURS presenting as acute abdomen with Right Iliac Fossa (RIF) mass 57-year-old male. Contrast Enhanced Computed Tomography...

purpose: this study evaluated the effect of donor and eye bank characteristics on graft rating and clinical outcomes after penetrating keratoplasty (pk) in keratoconus-affected patients. methods: a total of 252 consecutive keratoconic eyes that underwent pk were reviewed in this retrospective interventional case series. donor data included age and sex, cause of death, death-to-preservation time...

Background: Noscapine is cough suppressant drug with cell inhibitory effect. It is introduced for anti-cancer treatment. The aim of present study was to determine in vitro noscapine effect on nitric oxide (NO) secretion by human endometrial stromal cells. Materials and Methods: Human endometrial biopsies (n=8) were obtained in sterile condition and were chopped mechanically and digested by enzy...

The aim of this study was to isolate mouse embryonic stem cells from late blastocyst stage embryos and to use them as a model system for the study of hematopoietic induction outside the embryo by coculturing of embryonic stem cells with bone marrow stromal cells. Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 1-2 days in DMEM medium. The inner cell masses formed...

Background: Endometriosis is a complex disorder in reproductive age women which consist of stromal and epithelial cells implantation outside the uterine cavity. Adiponectin is a member of cytokine family with various metabolic roles and proliferation inhibition of many cancer cells. The aim of the present research was to determine adiponectin effect on human endometriotic stromal cells (ESCs) p...

background: non-melanoma skin cancer is the most common malignant tumor in humans. the role of ultraviolet radiation is well-known in the pathogenesis of basal cell carcinoma (bcc) and squamous cell carcinoma (scc). cd10 is a zinc-dependent metallopeptidase known as common acute lymphoblastic leukemia antigen (calla). although cd10 expression has been investigated in some cutaneous tumors, to o...