We have investigated the capacity of our established thymic stromal cell clone (MRL104.8a) or its derived factor(s) to induce the differentiation of immature thymocytes. Culture of purified adult murine double-negative (CD4-CD8-, indicated here as CD4-8-) thymocytes on the MRL104.8a thymic stromal cell monolayer for 1 day resulted in the induction of an appreciable percentage of CD4-8+ thymocyt...

carpal chip fractures are common causes of lameness in racehorses. due to disadvantages in surgical management, adjuvant treatment modalities are usually necessary. adipose-derived stem cells (adscs) have the potential to differentiate into other cell types including bone and cartilage cells. adipose-derived stromal vascular fraction (svf) is produced during adscs isolation from adipose tissue....

background: differentiation, migratory properties and availability of mesenchymal stromal cells (msc) have become an important part of biomedical research. however, the functional heterogeneity of cells derived from different tissues has hampered providing definitive phenotypic markers for these cells. objective: to characterize and compare the phenotype and cytokines of adipose derived mscs (a...

To examine the influence of the hematopoietic microenvironment on hematopoietic cell proliferation and differentiation during the yolk sac phase of hematopoiesis, we have recently established cell lines from embryonic yolk sac visceral endoderm (YSE) and mesoderm (YSM). In the present experiments, we compared in vitro growth of adult murine bone marrow high proliferative potential colony-formin...

We have studied a rapid cultivation method for human mesenchymal stromal cells based on CellGroTM medium and human serum, supplemented with insulin, ascorbic acid, dexamethasone, epidermal growth factor, platelet-derived growth factor BB, macrophage colony-stimulating factor and fibroblast growth factor 2. This study has shown that ...

The majority of human immunodeficiency virus (HIV)-seropositive patients develop bone marrow abnormalities associated with hematopoietic malfunction during the progression of disease. One important manifestation of HIV-associated hematopoietic dysfunction is that after myelosuppression, bone marrow recovery, a process known to be mediated in part by the production of stromal cell-derived hemato...