It was observed that in 24 hours following the injection of the labeled sulfate more than twice the amount of sulfur was found in intestine, stomach, liver and eyes of vitamin A supplemented rat, as compared with that in those tissues of the deficient one. When S35-methionine was administered, in contrast to the case of S35-sul fate, S35 concentration in tissues of control rat was significantly...

Fumarate hydratase (FH) is an enzyme of the tricarboxylic acid (TCA) cycle mutated in hereditary and sporadic cancers. Despite recent advances in understanding its role in tumorigenesis, the effects of FH loss on mitochondrial metabolism are still unclear. Here, we used mouse and human cell lines to assess mitochondrial function of FH-deficient cells. We found that human and mouse FH-deficient ...

The steady state concentrations of S-containing compounds formed in Lemna paucicostata Hegelm. 6746 in response to variations in source and concentrations of sulfur were measured. Neither growth rates nor protein accumulation were markedly affected by the various growth conditions. Ignoring complications due to possible compartmentation, the results are consistent with internal pools of both SO...

Tellurite exerts a deleterious effect on a number of small molecules containing sulfur moieties that have a recognized role in cellular oxidative stress. Because cysteine is involved in the biosynthesis of glutathione and other sulfur-containing compounds, we investigated the expression of Geobacillus stearothermophilus V cysteine-related genes cobA, cysK, and iscS and Escherichia coli cysteine...

Experiments on the formation of adenine thiomethylriboside from methionine in yeast (1) suggested a study of ethionine as a source of organic sulfur in the growth medium. The sulfur atom of this amino acid analogue is not utilized for the formation of thiomethyladenosine (2). However, we found in extracts of yeast (Torulopsis utilis and Saccharomyces cerevisiae) grown in the presence of an exce...

Two protein isolates (PI1 and PI2) were prepared from sunflower meal produced in Bulgaria. They were obtained by using isoelectric or ethanol precipitation following the extraction of the proteins with 10% NaCl. Their amino acid composition and water solubility as a function of pH and presence of NaCl were investigated. Lysine was established as the first limiting amino acid in the protein isol...

The iron-sulfur protein of the cytochrome bc1 complex is one of a small number of proteins that are processed in two sequential steps by matrix processing peptidase (MPP) and mitochondrial intermediate peptidase (MIP) during import into Saccharomyces cerevisiae mitochondria. To test whether two-step processing is necessary for import and assembly of the iron-sulfur protein into the cytochrome b...

The present study aims to assess the effect of Thiobacillus and sulfur on morphological and biochemical properties of medicinal pumpkinproperties under deficit irrigation based on a split-plot design. For this purpose, water stress was applied as a main factor in three levels (control, no irrigation in flowering stage, no irrigation in fruiting), and Thiobacillus and sulfur fertilizers as sub-p...