We have isolated a protein from media conditioned by a murine marrow-derived cell line (PB6) and from mouse marrow supernatants that antagonizes interleukin 3-dependent proliferation of cells in culture and reversibly inhibits DNA synthesis of erythroid progenitor cells (BFU-E) in vitro. This protein, p16 (monomer Mr = 16 kD on SDS-PAGE), was purified to homogeneity and amino acid sequencing of...

Breast cancer is one of the most commonest cancer after the cervical cancer in Indian women. The balance between oxidant and antioxidant has been suggested as an important factor for initiation and progression of breast cancer. The aim of the study is to determine erythrocyte superoxide dismutase (SOD), catalase (CAT) activities as antioxidant enzymes and malondialdehyde (MDA) levels in women h...

The oncogenic ras protein controls signal-transduction pathways that are critical for cell proliferation and tumorigenesis. Here, we demonstrate that v-Ha-ras-transduced human keratinocyte HaCaT cells produced significantly larger amounts of superoxide than did control cell lines. The superoxide generation was mediated by the transduced ras protein, because superoxide generation was modified by...

Human retinal pigment epithelium (RPE) contains two genetically distinct forms of superoxide dismutase (SOD) enzymes that scavenge harmful superoxide anions. Biochemical and immunochemical techniques were used to compare levels of copper-zinc- and manganese-containing forms of SOD (CuZn-SOD and Mn-SOD) in human adult and fetal RPE cells. It was found that Mn-SOD activity was higher in adult tha...

Superoxide dismutase (SOD) catalyzes the conversion of superoxide radical to hydrogen peroxide. Periplasmic localization of bacterial Cu,Zn-SOD has suggested a role of this enzyme in defense against extracellular phagocyte-derived reactive oxygen species. Sequence analysis of regions flanking the Salmonella typhimurium sodC gene encoding Cu,Zn-SOD demonstrates significant homology to lambda pha...

In recent years there is an upsurge in the areas related to newer developments in prevention of disease especially the role of free radicals and antioxidants. So it will be pertinent to examine the possible role of ‘free radicals’ in disease and ‘antioxidants’ in its prevention, especially the current status of the subject matter and future prospects. Numerous short-lived and highly reactive ox...

Copper/zinc superoxide dismutase (Cu/Zn SOD) is an essential enzyme that protects tissue from oxidative damage. Herein we report the first fluorogenic probe (SODO) for the detection and in vivo imaging of Cu/Zn SOD. SODO represents a unique chemical probe for translational imaging studies of Cu/Zn SOD in inflammatory disorders.

•2 Excessive production and/or inadequate removal of reactive oxygen species, especially superoxide anion (O ), have been implicated 2 in the pathogenesis of many cardiovascular diseases, including atherosclerosis, hypertension, diabetes, and in endothelial dysfunction by •2 decreasing nitric oxide (NO) bioactivity. Since the vascular levels of O are regulated by the superoxide dismutase (SOD) ...