This work shows that single sequencing reactions analyzed on an automated DNA sequencer can be an efficient way of screening PCR products for known mutations. We have analyzed a mutation in exon 10 of the human aromatase gene and show that an unambiguous genotype could be elucidated in more than 90% of the analyzed samples. Compared to analysis by full sequencing, 4 times more samples can be an...

A rapid and simplified fluorescence in situ hybridization (FISH) technique for the detection of human chromosome-specific centromeric probes is described. Using chromosomes 1-, 4-, 11and Y-specific fluorescence-labeled probes, the modified, or quick-FISH technique, was compared to two conventional FISH methods. The modified FISH technique detected human chromosomes without sacrificing sensitivi...

We describe a technique for arthroscopy of the wrist which is carried out without traction and with the arm lying horizontally on the operating table. The wrist is not immobilised, which makes it possible to assess the extent of instability after a ligamentous tear. In a prospective study of 30 patients we compared this technique with conventional wrist arthroscopy, performing the new method fi...

The clinico-pathological features of 133 consecutive cases of Henoch-Schönlein purpura are presented, with emphasis on the gastrointestinal manifestations. The potential pitfalls of contrast radiography are underlined with respect to management of intussusception and a plea is made to re-establish clinical assessment of the abdomen as the prime indicator in deciding to undertake laparotomy.

Background More minimally invasive techniques are currently available for the surgical oncologist in the optimal staging of biliopancreatic cancer. With the increased age of population we can see an increase of age-related diseases, such a cardiovascular disease, hypertension, arthritis and other malignancies. With the development of endoscopy, laparoscopy, ultrasonography and biopsy equipment,...

Background and Aim: Degenerative lumbar scoliosis is a spinal deformity resulting from advanced disc degeneration and facet arthropathy. Given the inconclusive available literature and lack of high-quality data supporting the role of minimally invasive surgical management of degenerative lumbar scoliosis, this review intends to highlight and compare the various viable minimally invasive surgica...

A new payment system, the diagnosis-related group (DRG) system, and Korean diagnosis procedure combination (KDPC, per-diem) payment system were officially introduced in 2002 and in 2012, respectively. We evaluated the impact of payment system change from per-case to per-diem on high severity patient's length of stay (LOS).Claim data was used. A total of 36,240 case admissions and 72,480 control...

In keeping with the Congressional Budget Office (CBO) definition of CE (“. . .a rigorous evaluation of the impact of different options that are available for treating a given medical condition for . . . a set of patients”) and the experience of several nations, the authors reviewed CE as applied to medical devices and drugs, surgical interventions, and diagnostic tests. Their examination focuse...