Chondronectin is a cartilage matrix protein that specifically mediates the attachment of chondrocytes to type II collagen. A monoclonal antibody to chondronectin was used in a competitive enzyme linked immunosorbent assay (ELISA) and Western blot assay to detect, measure, and characterise chondronectin in human synovial fluid. Chondronectin was detected in all 18 rheumatoid and 11 osteoarthriti...

Uric acid (UA) is known to activate the NLRP3 (Nacht, leucine-rich repeat and pyrin domain containing protein 3) inflammasome. When activated, the NLRP3 (also known as NALP3) inflammasome leads to the production of IL-18 and IL-1β. In this cohort of subjects with knee osteoarthritis (OA), synovial fluid uric acid was strongly correlated with synovial fluid IL-18 and IL-1β. Synovial fluid uric a...

The study consisted of 18 dogs divided into Three groups. Each group consisted of Six dogs with elbow, hip and stifle joint disorders respectively. All the dogs were subjected to synovial fluid evaluation. The physico-chemical parameters recorded in these dogs included synovial fluid volume, color, viscosity, transparency, protein concentration, total nucleated cell count and differential cell ...

The dimethylmethylene blue assay showed higher concentrations of glycosaminoglycans in many synovial fluids from patients with rheumatoid arthritis (RA) than in autologous sera or sera or synovial fluids from normal subjects. These results were taken to suggest that the glycosaminoglycans in RA synovial fluid were abnormally raised and derived from cartilage. To discover what stimulated such gl...

The composition of synovial fluid in rheumatoid arthritis (RA) is complex and strongly influences the microenvironment of joints and it is an inseparable element of the disease. Currently, "in vitro" studies are performed on RA cells cultured in the presence of either recombinant proinflammatory cytokines-conditioned medium or medium alone. In this study, we evaluated the use of synovial fluid,...

OBJECTIVE Pentosidine, an advanced glycation end product (AGE), has recently been observed to be elevated in rheumatoid arthritis (RA). The aim was to elucidate which pentosidine levels, i.e. in serum, synovial fluid or urine, are more related to the disease status of RA. METHODS We measured levels of pentosidine in serum, synovial fluid or urine in RA compared with osteoarthritis (OA), and e...

Interplay of the constituents of the articular cartilage synovial fluid combine and its role was examined through the biochemical and rheological studies on bovine joints. The results showed an inverse relationship between the changes in the hyaluronic acid of synovial fluid and the proteoglycans content in articular cartilage together with alterations in the rheological properties of synovial ...

Multicentric reticulohistiocytosis (MR) is a systemic disease of unknown cause characterized by the presence of a heavy macrophage infiltrate in skin and synovial tissues and the development of an erosive polyarthritis. The synovial fluid in MR is known to contain numerous mononuclear cells. In this study, we have determined whether these cells contribute to joint destruction in MR by different...