Toxoplasma gondii is a protozoan parasite with a broad range of intermediate hosts. Chickens as important food-producing animals can also serve as intermediate hosts. To date, experimental studies on the pathogenicity of T. gondii in broiler chickens were rarely reported. The objective of the present study was to compare the pathogenicity of 5 different T. gondii strains (RH, CN, JS, CAT2, and ...

Infection of mouse macrophages with Toxoplasma gondii elicits MAPK activation and IL-12 production, but host cell signaling pathways have not been clearly delineated. Here, we compared macrophage signaling in response to high virulence type I (RH) vs low virulence type II (ME49) strain infection. Tachyzoites of both strains induced p38 MAPK-dependent macrophage IL-12 release, although ME49 elic...

this study was aimed to detect anti-toxoplasma gondii antibodies in cats infected with rh strain of t. gondii by using an immunoblotting method. for this, cats were experimentally infected using tachyzoites harvested from vero cell cultures. tachyzoites were then lysed and transferred to polyacrylamide gels followed by blotting to pvdf membranes. an immunoblotting was performed using these memb...

Toxoplasma gondii is an obligate intracellular protozoan parasite found worldwide that is able to chronically infect almost all vertebrate species, especially birds and mammalians. Chitinases are essential to various biological processes, and some pathogens rely on chitinases for successful parasitization. Here, we purified and characterized a chitinase from T. gondii. The enzyme, provisionally...

The ability of Toxoplasma gondii tachyzoites to differentiate into latent bradyzoite forms is essential for pathogenesis of clinical disease. We examined the effects of cyclic nucleotides on T. gondii bradyzoite differentiation in vitro. Differentiation of tachyzoites to bradyzoites was measured in an immunofluorescence assay using ME49 or its clonal derivative PLK, two well-characterized T. go...

BACKGROUND Toxoplasmosis is considered as one of the most common infectious diseases caused by the protozoan parasite Toxoplasma gondii. Tachyzoite is the main form of Toxoplasma and continuously is maintained in cell culture or injected into the mice peritoneal cavity. This study was designed to evaluate the survival rate of RH strain of T. gondii tachyzoites in different cell free, nutrient a...

PURPOSE Retinochoroidal infection with the protozoan parasite Toxoplasma gondii is the most common cause of posterior uveitis worldwide. Tachyzoites spread throughout the body through the blood stream and lymphatics, but preferentially encyst in the eye and other parts of the central nervous system (CNS). It is unknown whether T. gondii penetrates the CNS selectively or whether these sites of i...

background: this study was undertaken to evaluate the viability, infectivity and immunity of toxoplasma gondii tachyzoites exposed to 2-(naphthalene-2-ylthio)-1h-indole.   methods : tachyzoites of rh strain were incubated in various concentrations of 2- (naphthalene-2-ylthio)-1h-indole (25-800μm) for 1.5 hours. then, they were stained by pi and analyzed by fluorescence-activated cell sorting (f...

background: toxoplasmosis is considered as one of the most common infectious diseases caused by the protozoan parasite toxoplasma gondii . tachyzoite is the main form of toxoplasma and continuously is maintained in cell culture or injected into the mice peritoneal cavity. this study was designed to evaluate the survival rate of rh strain of t. gondii tachyzoites in different cell free, nutrient...

BACKGROUND This study was undertaken to evaluate the viability, infectivity and immunity of Toxoplasma gondii tachyzoites exposed to 2-(naphthalene-2-ylthio)-1H-indole. METHODS Tachyzoites of RH strain were incubated in various concentrations of 2-(naphthalene-2-ylthio)-1H-indole (25-800 μM) for 1.5 hours. Then, they were stained by PI and analyzed by Fluorescence-activated cell sorting (FACS...