With the aid of brownian dynamics models, it is possible to estimate polymer tension by monitoring polymers' transverse thermal fluctuations. To assess the precision of the approach, brownian dynamics-based tension estimates were compared with the force applied to rhodamine-phalloidin labeled actin filaments bound to polymer beads and suspended between two optical traps. The transverse thermal ...

BACKGROUND The spatiotemporal regulation of adhesion to the extracellular matrix is important in metazoan cell migration and mechanosensation. Although adhesion assembly depends on intracellular and extracellular tension, the biophysical regulation of force transmission between the actin cytoskeleton and extracellular matrix during this process remains largely unknown. RESULTS To elucidate th...

A rigorous description of the magnitude and direction of the 3D force vector each thumb muscle produces at the thumb-tip is necessary to understand the biomechanical consequences to pinch of a variety of paralyses and surgical procedures (such as tendon transfers). In this study, we characterized the 3D force vector each muscle produces at the thumb-tip, and investigated if these thumb-tip forc...

Cable tension force is one of the most important structural parameters to monitor in cable-stayed bridges. For example, cable tension needs to be monitored during construction and maintenance to ensure the bridge is not overloaded. To economically monitor tension forces, this study proposes the use of an automated wireless tension force estimation system (WFTES) developed solely for cable force...

Focal adhesions (FAs) are the predominant mechanism by which cells mechanically couple to and exert traction forces on their extracellular matrix (ECM). It is widely presumed that FA size is modulated by force to mediate changes in adhesion strength at different levels of cellular tension. However, previous studies seeking correlations between force and FA morphology have yielded variable and o...

The length-tension relationship is a fundamental property of muscle. In its classic form, which is used in muscle models incorporated into studies of motor control, the length-tension relationship is measured during maximal activation via tetanic electrical stimulation in whole muscles or during high intracellular calcium levels in single muscle fibers. In this study, we measured the length-ten...

Ten pairs of cat papillary muscles were arranged in tandem to study their individual and combined length-tension and force-velocity relationships. Physiologic asynchronous contraction (up to 60 msec) of the pair of muscles produced a change in the individual force-velocity relations of each muscle which was characteristic of a change in muscle length; i.e., there was an alteration of tension de...

Effects of sustained postextrasystolic potentiation (P.S.), norepinephrine (NE), and calcium on resting length-tension relations of heart muscle were studied in the cat papillary muscle and isovolumic dog ventricle. In papillary muscles, P.S., calcium or NE produced no change in diastolic compliance when the muscles were freely isotonic, or afterloaded, and force remained constant. However, und...

Adherent cells interact with extracellular matrix via cell-substrate contacts at focal adhesions. The dynamic assembly and disassembly of focal adhesions enables cell attachment, migration and growth. While the influence of mechanical forces on the formation and growth of focal adhesions has been widely observed, the force loading on specific proteins at focal adhesion complex is not clear. By ...