The two-step process of selection and counter-selection is a standard way to enable genetic modification and engineering of bacterial genomes using homologous recombination methods. The tetA and sacB genes are contained in a DNA cassette and confer a novel dual counter-selection system. Expression of tetA confers bacterial resistance to tetracycline (Tc(R)) and also causes sensitivity to the li...

BACKGROUND Dietary zinc oxide is used in pig nutrition to combat post weaning diarrhoea. Recent data suggests that high doses (2.5 g/kg feed) increase the bacterial antibiotic resistance development in weaned pigs. Therefore, the aim of this study was to investigate the development of enterobacterial antibiotic resistance genes in the intestinal tract of weaned pigs. FINDINGS Weaned pigs were...

Citation: Du X, He F, Shi Q, Zhao F, Xu J, Fu Y and Yu Y (2018) The Rapid Emergence of Tigecycline Resistance in blaKPC−2 Harboring Klebsiella pneumoniae, as Mediated in Vivo by Mutation in tetA During Tigecycline Treatment. Front. Microbiol. 9:648. doi: 10.3389/fmicb.2018.00648 The Rapid Emergence of Tigecycline Resistance in blaKPC−2 Harboring Klebsiella pneumoniae, as Mediated in Vivo by Mut...

leu-500 is a chromosomal promoter mutation in Salmonella typhimurium that normally causes the promoter to be inactive in the initiation of RNA synthesis. But in a strain that has mutations in topA, the gene encoding DNA topoisomerase I, the mutant promoter becomes active. We show that the leu-500 promoter can function on a plasmid when it is adjacent to the tetracycline-resistance gene tetA. Ac...

Purpose: Somatostatin receptors (SSTr) are expressed on many neuroendocrine tumors, and several radiotracers have been developed for imaging these types of tumors. For this reason, peptide analogues of somatostatin have been well characterized. Copper-64 (t1/2 12.7 hours), a positron emitter suitable for positron emission tomography (PET) imaging, was shown recently to have improved in vivo cle...

In 1995, Dell and Teta published a cohort mortality study of asbestos molding compound workers at a Union Carbide Corporation (UCC) plastics manufacturing plant in Bound Brook, New Jersey. They reported that the factory workers were exposed to "asbestos (mostly chrysotile)," implying that the asbestos used at the Bound Brook plant occasionally contained amphiboles. However, UCC statements and t...

El problema a resolver es mantener siempre en posición vertical la masa junto con la varilla, tratando de mover el carro para lograrlo. El péndulo esta sostenido por una chumacera, encima de un carro que a su vez tiene movimiento por medio de un sistema de deslizamiento dinámico, por lo cual éste puede adoptar dos tipos de direcciones sobre el plano x, y que afecta directamente con el ángulo te...

To determine whether microbial chemosensors can be used to find new or better biocatalysts, we constructed Escherichia coli hosts that recognize the product of a biocatalytic conversion through the transcriptional activator NahR and respond by expression of a lacZ or tetA reporter gene. Equipped with a benzaldehyde dehydrogenase (XylC from Pseudomonas putida), the lacZ-based host responded to t...

Abstract In the emerging networked sensor systems, collaborative in-network processing provides a viable solution to overcome the limited energy and resource constraints of one single node. In this novel computing paradigm, it is very critical to perform task assignment. In this paper, we formally model TETA, an energy efficient topology-aware real time task assignment problem in wireless senso...