Objective. To determine the freezing and thawing rates necessary to maintain sperm viability during cryopreservation of Bocachico semen. Materials and methods. Four interactional treatments were implemented between two freezing (rapid and slow) and two thawing (rapid and slow) curves, in a 2x2 factorial as follows: rapid freezing-rapid thawing, rapid freezing-slow thawing, slow freezingrapid th...

Preservation of bacteria at the temperature of dry ice (-78.5 C) is a particularly valuable method for the maintenance of such organisms as Treponema pallidum which otherwise require continuous animal-to-animal passage. Turner (1938), Turner and Fleming (1939), and Turner and Brayton (1939) demonstrated that at the temperature of dry ice T. pallidum and relapsing fever spirochetes retain their ...

Polymerase chain reaction (PCR) is now widely used in malaria research for analysis of field samples. However, little has been reported regarding loss of sensitivity due to field methodology. Therefore, studies were carried out in relation to blood sampling (anticoagulants, culture medium, filter paper), storage (temperature, time and immediate lysis) and handling (repeated thawing and freezing...

BACKGROUND The aim of this study was to perform validation of the thawing process for solvent/detergent-treated plasma octaplasLG® using different thawing devices. Optimized settings for temperature and thawing time should be defined based on the results of both temperature measurements and extensive biochemical characterization studies. METHODS octaplasLG units were thawed using water bath s...

Permafrost thawing may lead to the release of carbon and nitrogen in high-latitude regions Northern Hemisphere, mainly form greenhouse gases. Our research aims reveal effects permafrost on CH4 N2O emissions from peatlands Xiaoxing’an Mountains, Northeast China. During four growing seasons (2011–2014), situ were monitored peatland under no-thawing, mild-thawing, severe-thawing conditions middle ...

BACKGROUND Fresh frozen plasma (FFP) should be thawed before transfusing to the patient. Prolonged or uncontrolled thawing can denature plasma proteins. The potential risk of contamination by wet thawing had always been a point of concern. AIMS Here, we compared and evaluated the effect of thawing on clotting factor activities by two different methods (wet and dry) and other factors such as r...

In four experiments, the effects of pre-thaw delay, method of thawing, temperature of thawing solution and pellet volume on acrosome morphology and moti l i ty of" boar spermatozoa frozen by the pellet method were examined. Holding the frozen pellets for 0, 1.5, 3 or 4.5 min in a Styrofoam container before transferring them into the thawing solution resulted in maximum post-thaw percentages of ...

In this study,the quality characteristics which include(physicochemical,structural and sensory evaluation)of beef loin submitted to fast freezing thawed by five different methods (Refrigerator,Oven,Microwave,tap water Room temperature)were examined The samples were defrosted the point of reaching a core temperature 0oC,followed several parameters include physical characteristics[(drip loss,thaw...

The objective of this study was to determine the effects of thawing groups of 2, 5, 10, 15, or 20 .5-ml French straws on post-thaw spermatozoal viability. Thermostatically controlled and nonthermostatically controlled thawing baths were compared. Using a split-plot design, semen from 10 bulls was extended in egg yolk citrate, frozen, and then thawed (in the respective groups) at 36 degrees C in...

The objective of this study was to determine the effect of freezing and thawing or freezing and thawing with an additional aging period after frozen storage on the tenderness of longissimus lumborum (LL) and semitendinosus (ST) steaks relative to aged, fresh steaks. Left-side LL and ST (n = 35 each) were obtained from U.S. Select carcasses classified at the grading stand by the U.S. Meat Animal...