Although SARS-CoV-2-neutralizing antibodies are promising therapeutics against COVID-19, little is known about their mechanism(s) of action or effective dosing windows. We report the generation and development SC31, a potent SARS-CoV-2 neutralizing antibody, isolated from convalescent patient. Antibody-mediated neutralization occurs via an epitope within receptor-binding domain Spike protein. S...

introduction: addiction is a habit or behavior that is often hard to quit. it is estimated that 190 million persons are substance users around the world. substance abuse creates tolerance after a while. shortly after taking the drug, a person will not enjoy as the beginning of its use that is why the amount of substance used is gradually increased. the purpose of this study was to find the reas...

Background: Myocardial infarction (MI) is one of the most common and serious diseases resulting from coronary artery occlusion and major reduction in blood flow. Streptokinase as a thrombolytic is considered the first and most important therapeutic intervention for reperfusion following MI in most countries including Iran. Our previous study showed that, the prevalence of high antibody titers a...

Introduction: Gastric cancer, as the second reason of cancer mortality around the world, is defined as one of the serious health problems and a wide range of current research is focused to overcome the challenges relevant to its successful therapy. Employing recent approaches in the field of vaccine tumor therapy and immunotherapic strategies such as utilization of monoclonal antibodies can pla...

Background: Mesothelin is a tumor differentiation antigen; its binding to tumor-antigen CA-125 can lead to tumor metastasis. Results: Structures of the Fab from a therapeutic antibody MORAb-009 and its complex with an epitope-containing fragment of mesothelin are obtained. Conclusion: Overlapping binding sites for both CA-125 and MORAb-009 provides a basis for the antibody’s therapeutic effect....

I n today’s biopharmaceutical pipeline, monoclonal antibodies are a predominant modality for a broad range of clinical indications, including inflammatory disorders, oncology, and infectious diseases.1 More than two dozen antibody-based products are commercially available. In 2004, six of the 12 new biopharmaceutics that gained approval in the United States and Europe were antibody-based produc...

Glycosylation patterns in antibodies critically determine biological and physical properties but their precise control is a significant challenge in biology and biotechnology. We describe herein the optimization of an endoglycosidase-catalyzed glycosylation of the best-selling biotherapeutic Herceptin, an anti-HER2 antibody. Precise MS analysis of the intact four-chain Ab heteromultimer reveals...

Immunohistochemistry (IHC) is an ancillary method, widely used in pathologist practice, that allows to identify diagnostic and prognostic/predictive therapeutic response protein markers on tissue samples by the use of specific monoclonal antibodies chromogenic substances guarantee visualization antibody–antigene binding complex under light microscope [...]