OBJECTIVE To investigate the prevalence of autoimmune thyroid disease in diabetic children in Northwest Iran. METHODS In a cross-sectional study from February 2006 to November 2007, serum levels of anti-thyroid peroxidase, and anti-thyroglobulin antibodies, and thyrotropin hormone were measured with ELISA method in 176 diabetic children (78 Male and 98 Female) at a mean age of 8.3 +/- 3.7 and...

It is also known that RAI treatment can induce an alteration in immune response to thyroid antigens in Graves’ disease (5). Thus it is also possible that thyroid antigens can be released into the circulation after destruction of thyroid cells by RAI in patients with thyroid cancer. Evidence for this comes from our previous study which showed that serum thyroid peroxidase was detectable after RA...

Organ specific autoimmune diseases are relatively common immunological disorders in man which include thyroid autoimmune disease, insulin-dependent diabetes mellitus and myasthenia gravis. The target autoantigens in some of these diseases have recently been characterised. In thyroid autoimmune disease this includes the key enzyme, thyroid peroxidase (TPO), which is involved in the generation of...

The one- or two-electron oxidation of thyroglobulin by the thyroid peroxidase system was found to be regulated by the iodine content of thyroglobulin. The catalytic intermediate of thyroid peroxidase observed at steady state of the reaction was Compound I and II when the iodine content in thyroglobulin was 0.2 and 0.7%, respectively, apparent rate constants for the rate-limiting steps being est...

The kinetics of iodination and oxidation of hog thyroglobulin were studied with purified hog thyroid peroxidase and the results were compared with the reactions of free tyrosine. From Lineweaver-Burk plots and on the basis of a value of 0.83 for delta epsilon mM at 289 nm/iodine atom incorporated, the rate constant for transfer of an assumed enzyme-bound iodinium cation to thyroglobulin was est...

Mechanisms that have been proposed for peroxidase-catalyzed iodination require the utilization of 1 mol of H2O2 for organic binding of 1 mol of iodide. When we measured the stoichiometry of this reaction using thyroid peroxidase or lactoperoxidase at pH 7.0, we consistently obtained a ratio less than 1.0. This was shown to be attributable to catalase-like activity of these enzymes, resulting in...

Autoimmune thyroid diseases (AITD) are characterized by the presence of thyroid-specifi c autoantibodies. These are direc ted against thyroid microsomes, for which the main target antigen is thyroid peroxidase (TPO), against thyroglobulin (TG), or against the thyroid stimulating hormone (TSH) receptor. AITD frequently occur in combination with other autoimmune diseases, such as myasthenia gravi...

Autoimmune thyroid diseases (AITD) are characterized by the presence of thyroid-specifi c autoantibodies. These are direc ted against thyroid microsomes, for which the main target antigen is thyroid peroxidase (TPO), against thyroglobulin (TG), or against the thyroid stimulating hormone (TSH) receptor. AITD frequently occur in combination with other autoimmune diseases, such as myasthenia gravi...